Molecular species identification for multiple carnivores
Species identification is crucial for carnivore conservation and ecological studies. We present a simple molecular genetic test that amplifies DNA of 16 wild carnivore species from three continents. The test is based on co-amplification of two mitochondrial DNA fragments and scoring of the resulting...
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| Format: | article |
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2017
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| Accés en línia: | http://dx.doi.org/10.1007/s12686-014-0257-x http://dspace.utpl.edu.ec/handle/123456789/19072 |
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Afegir etiqueta | _version_ | 1858364504848465920 |
|---|---|
| author | Cisneros Vidal, R. |
| author_facet | Cisneros Vidal, R. |
| author_role | author |
| collection | Repositorio Universidad Técnica Particular de Loja |
| dc.creator.none.fl_str_mv | Cisneros Vidal, R. |
| dc.date.none.fl_str_mv | 01/12/2014 2017-06-16T22:02:53Z 2017-06-16T22:02:53Z |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.1007/s12686-014-0257-x 1877-7252 http://dx.doi.org/10.1007/s12686-014-0257-x http://dspace.utpl.edu.ec/handle/123456789/19072 |
| dc.language.none.fl_str_mv | Inglés |
| dc.publisher.none.fl_str_mv | CONSERVATION GENETICS RESOURCES |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Técnica Particular de Loja instname:Universidad Técnica Particular de Loja instacron:UTPL |
| dc.subject.none.fl_str_mv | carnivores fragment analysis mitochondrial dna control region non invasive genetic samples species identification |
| dc.title.none.fl_str_mv | Molecular species identification for multiple carnivores |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Species identification is crucial for carnivore conservation and ecological studies. We present a simple molecular genetic test that amplifies DNA of 16 wild carnivore species from three continents. The test is based on co-amplification of two mitochondrial DNA fragments and scoring of the resulting species-specific size patterns. We evaluated the performance of this method using 332 known tissue, blood, hair and fecal samples from 23 carnivore and 11 potential prey species. Results demonstrate that this test can distinguish many Caniform species but not members of Felidae. The test can be performed with a single PCR and capillary sequencer run for cost-effective processing of large sample numbers typical of non-invasive genetic projects. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UTPL_d4fdce49b4226900f15dd2fb4dbfb73f |
| identifier_str_mv | 1877-7252 |
| instacron_str | UTPL |
| institution | UTPL |
| instname_str | Universidad Técnica Particular de Loja |
| language_invalid_str_mv | Inglés |
| network_acronym_str | UTPL |
| network_name_str | Repositorio Universidad Técnica Particular de Loja |
| oai_identifier_str | oai:dspace.utpl.edu.ec:123456789/19072 |
| publishDate | 2017 |
| publisher.none.fl_str_mv | CONSERVATION GENETICS RESOURCES |
| reponame_str | Repositorio Universidad Técnica Particular de Loja |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Técnica Particular de Loja - Universidad Técnica Particular de Loja |
| repository_id_str | 1227 |
| spelling | Molecular species identification for multiple carnivoresCisneros Vidal, R.carnivoresfragment analysismitochondrial dna control regionnoninvasive genetic samplesspecies identificationSpecies identification is crucial for carnivore conservation and ecological studies. We present a simple molecular genetic test that amplifies DNA of 16 wild carnivore species from three continents. The test is based on co-amplification of two mitochondrial DNA fragments and scoring of the resulting species-specific size patterns. We evaluated the performance of this method using 332 known tissue, blood, hair and fecal samples from 23 carnivore and 11 potential prey species. Results demonstrate that this test can distinguish many Caniform species but not members of Felidae. The test can be performed with a single PCR and capillary sequencer run for cost-effective processing of large sample numbers typical of non-invasive genetic projects.CONSERVATION GENETICS RESOURCES2017-06-16T22:02:53Z2017-06-16T22:02:53Z01/12/2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12686-014-0257-x1877-7252http://dx.doi.org/10.1007/s12686-014-0257-xhttp://dspace.utpl.edu.ec/handle/123456789/19072Inglésinfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Técnica Particular de Lojainstname:Universidad Técnica Particular de Lojainstacron:UTPL2017-06-16T22:02:53Zoai:dspace.utpl.edu.ec:123456789/19072Institucionalhttps://dspace.utpl.edu.ec/Institución privadahttps://www.utpl.edu.ec/https://dspace.utpl.edu.ec/oai.Ecuador...opendoar:12272017-06-16T22:02:53Repositorio Universidad Técnica Particular de Loja - Universidad Técnica Particular de Lojafalse |
| spellingShingle | Molecular species identification for multiple carnivores Cisneros Vidal, R. carnivores fragment analysis mitochondrial dna control region non invasive genetic samples species identification |
| status_str | publishedVersion |
| title | Molecular species identification for multiple carnivores |
| title_full | Molecular species identification for multiple carnivores |
| title_fullStr | Molecular species identification for multiple carnivores |
| title_full_unstemmed | Molecular species identification for multiple carnivores |
| title_short | Molecular species identification for multiple carnivores |
| title_sort | Molecular species identification for multiple carnivores |
| topic | carnivores fragment analysis mitochondrial dna control region non invasive genetic samples species identification |
| url | http://dx.doi.org/10.1007/s12686-014-0257-x http://dspace.utpl.edu.ec/handle/123456789/19072 |