Detection of Influenza A virus, Respiratory syncytial virus and Human metapneumovirus in guinea pigs (Cavia porcellus) raised as livestock in Paute, Ecuador

Viral agents such as Influenza A virus, Respiratory syncytial virus and Human metapneumovirus are some of the most frequent human respiratory pathogens and a concern for the public health. Guinea pigs (Cavia porcellus) could be a potential reservoir of these pathogens, because their susceptibility to be inoculated by these microorganisms which has been mainly demonstrated in laboratory conditions. However, it is imperative to make diagnoses from samples obtained from animals raised as livestock, to confirm or ruled out a possible role of the guinea pig as a zoonotic vector. In countries such as Ecuador, where the annual production of these animals is considerably high, this type of studies could have greater relevance for public health. In the present investigation, eighty samples of nasopharyngeal washing of guinea pigs from the Paute canton in the province of Azuay were obtained. Diagnoses of the respiratory viruses Influenza A virus, Respiratory syncytial virus and Human metapneumovirus were made, through protocols known as One Step RT PCR and Two Step RT PCR. All the tests yield negative results for the three viruses analyzed and, therefore, the viruses were not isolated.

• Hovedforfatter: Rodríguez Pazmiño, Ángel Sebastián (author)
• Format: bachelorThesis
• Sprog: eng
• Udgivet: 2019
• Fag: Virus de Influenza A; Virus sincitial respiratorio; Metapneumovirus humano; Cavia porcellus; Cuyes; Infecciones respiratorias; Guinea pig; Respiratory infections
• Online adgang: http://repositorio.yachaytech.edu.ec/handle/123456789/102