Effects of Leukocyte-Platelet Rich Fibrin Membranes on Cell Proliferation and Cell Migration.

In regenerative medicine, repair and regeneration of defects and damage in both soft and hard tissues still remain a challenge Hence better bioengineering strategies and biomaterials are needed to meet the actual needs of this demanding area of the regenerative medicine. Autologous platelet concentrates are potential tools from which regenerative medicine can benefit. The Leukocyte-Platelet Rich Fibrin (L-PRF) is a sub-family of the PRF concentrates that has been in the spotlight because of its simplicity, and the presence in its enriched solid three-dimensional fibrin matrix of leukocytes, proteins, cytokines, growth factors, and stem cells. Although this biomaterial has produced excellent results in medical procedures, it is still unclear its exact mechanism of therapeutic action. In the present project, cell performance on L-PRF will be evaluated in vitro to explain some of the functionalizing of L-PRF on human dermal fibroblast (HDFn) and pre-osteoblasts (MC3T3-E1) cells. In addition, leukocyte effects on HDFn cells were registered for analyzes. Results for pre-cultured cells were >280% for fluorescence measures; and results for cells cultured at the same time with L-PRF were 2500% for absorbance measures. This was only achieved after performing PrestoBlue™ tests with the L-PRF membrane present on the well, this protocol also confirms that cells migrate towards the fibrin network of the L-PRF membrane. L-PRF showed to be an effective biomaterial that has many potentials to assist in the dental field with bone and soft tissue regeneration.

• Autor principal: Alcívar Carmigniani, Andrés Sebastián (author)
• Format: bachelorThesis
• Idioma: eng
• Publicat: 2020
• Matèries: L-PRF; Platelet concentrates; Cell proliferation; Cell migration; Concentrados de plaquetas; Proliferación celular; Migración celular
• Accés en línia: http://repositorio.yachaytech.edu.ec/handle/123456789/278