Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models

Currently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, t...

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Egile nagusia: Bonilla Vázquez, Alicia del Rocío (author)
Formatua: bachelorThesis
Hizkuntza:eng
Argitaratua: 2023
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Sarrera elektronikoa:http://repositorio.yachaytech.edu.ec/handle/123456789/692
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author Bonilla Vázquez, Alicia del Rocío
author_facet Bonilla Vázquez, Alicia del Rocío
author_role author
collection Repositorio Universidad Yachay Tech
dc.contributor.none.fl_str_mv Briceño Araujo, Sarah Elisa
Spencer Valero, Lilian Maritza
dc.creator.none.fl_str_mv Bonilla Vázquez, Alicia del Rocío
dc.date.none.fl_str_mv 2023-12-07T10:04:20Z
2023-12-07T10:04:20Z
2023-12
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://repositorio.yachaytech.edu.ec/handle/123456789/692
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Universidad de Investigación de Tecnología Experimental Yachay
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Yachay Tech
instname:Universidad Yachay Tech
instacron:Yachay
dc.subject.none.fl_str_mv Biotintas
Hidrogeles
Bioimpresión 3D
Bioink
Hydrogels
3D bioprinting
dc.title.none.fl_str_mv Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
description Currently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, these models can be generated that help recreate tumor environments, allowing the behavior of drugs to be studied. Therefore, the aim is to synthesize bioinks with characteristics such as biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and mechanical integrity. The present work studies hydrogels’ viability, morphology, and composition based on alginate and gelatin crosslinked with CaCl2 and genipin. The hydrogels were synthesized based on natural polymers such as sodium alginate and gelatin using ionic and natural crosslinking methods. The samples were characterized using ATR-FTIR, SEM, and MTT assay for cell viability. The obtained results revealed that the Caco-2 and HUVEC cell lines with which a tissue model can be developed to evaluate colorectal cancer drugs showed an improvement of the results in the gelatin, alginate, and CaCl2 hydrogels (68.92%) and the gelatin, alginate hydrogels with genipin at 0.25% w/v (60% in HUVEC and 75.07% in Caco-2). The results obtained demonstrate the potential use of alginate/gelatin bioinks for 3D human tissue models.
eu_rights_str_mv openAccess
format bachelorThesis
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oai_identifier_str oai:repositorio.yachaytech.edu.ec:123456789/692
publishDate 2023
publisher.none.fl_str_mv Universidad de Investigación de Tecnología Experimental Yachay
reponame_str Repositorio Universidad Yachay Tech
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Yachay Tech - Universidad Yachay Tech
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spelling Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue modelsBonilla Vázquez, Alicia del RocíoBiotintasHidrogelesBioimpresión 3DBioinkHydrogels3D bioprintingCurrently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, these models can be generated that help recreate tumor environments, allowing the behavior of drugs to be studied. Therefore, the aim is to synthesize bioinks with characteristics such as biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and mechanical integrity. The present work studies hydrogels’ viability, morphology, and composition based on alginate and gelatin crosslinked with CaCl2 and genipin. The hydrogels were synthesized based on natural polymers such as sodium alginate and gelatin using ionic and natural crosslinking methods. The samples were characterized using ATR-FTIR, SEM, and MTT assay for cell viability. The obtained results revealed that the Caco-2 and HUVEC cell lines with which a tissue model can be developed to evaluate colorectal cancer drugs showed an improvement of the results in the gelatin, alginate, and CaCl2 hydrogels (68.92%) and the gelatin, alginate hydrogels with genipin at 0.25% w/v (60% in HUVEC and 75.07% in Caco-2). The results obtained demonstrate the potential use of alginate/gelatin bioinks for 3D human tissue models.Actualmente, la probabilidad de que un fármaco en tratamientos contra el cáncer pase los ensayos clínicos es baja porque se prueban en modelos animales o cultivos celulares 2D, que no proporcionan un sistema preciso. Los investigadores han visto los modelos de tejidos 3D como una alternativa para la investigación oncológica ya que, con la bioimpresión 3D, se pueden generar estos modelos que ayudan a recrear entornos tumorales, permitiendo estudiar el comportamiento de los fármacos. Por tanto, el objetivo es sintetizar biotintas con características como biocompatibilidad, biodegradabilidad, no toxicidad, no inmunogenicidad e integridad mecánica. El presente trabajo estudia la viabilidad, morfología y composición de hidrogeles basados en alginato y gelatina entrecruzados con CaCl2 y genipina. Los hidrogeles se sintetizaron a partir de polímeros naturales como el alginato de sodio y la gelatina utilizando métodos de reticulación iónicos y naturales. Las muestras se caracterizaron mediante ensayos ATR-FTIR, SEM y MTT para determinar la viabilidad celular. Los resultados obtenidos revelaron que las líneas celulares Caco-2 y HUVEC con las que se puede desarrollar un modelo de tejido para evaluar fármacos contra el cáncer colorrectal mostraron una mejora de los resultados en los hidrogeles de gelatina, alginato y CaCl2 (68,92%) y en la gelatina, alginato. hidrogeles con genipina al 0,25% p/v (60% en HUVEC y 75,07% en Caco-2). Los resultados obtenidos demuestran el uso potencial de biotintas de alginato/gelatina para modelos 3D de tejido humano.Ingeniero/a Biomédico/aUniversidad de Investigación de Tecnología Experimental YachayBriceño Araujo, Sarah ElisaSpencer Valero, Lilian Maritza2023-12-07T10:04:20Z2023-12-07T10:04:20Z2023-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://repositorio.yachaytech.edu.ec/handle/123456789/692enginfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Yachay Techinstname:Universidad Yachay Techinstacron:Yachay2025-07-08T17:48:37Zoai:repositorio.yachaytech.edu.ec:123456789/692Institucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oaiEcuador...opendoar:102842025-07-08T17:48:37falseInstitucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oai.Ecuador...opendoar:102842025-07-08T17:48:37Repositorio Universidad Yachay Tech - Universidad Yachay Techfalse
spellingShingle Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
Bonilla Vázquez, Alicia del Rocío
Biotintas
Hidrogeles
Bioimpresión 3D
Bioink
Hydrogels
3D bioprinting
status_str publishedVersion
title Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
title_full Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
title_fullStr Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
title_full_unstemmed Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
title_short Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
title_sort Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
topic Biotintas
Hidrogeles
Bioimpresión 3D
Bioink
Hydrogels
3D bioprinting
url http://repositorio.yachaytech.edu.ec/handle/123456789/692