Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models

Currently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, these models can be generated that help recreate tumor environments, allowing the behavior of drugs to be studied. Therefore, the aim is to synthesize bioinks with characteristics such as biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and mechanical integrity. The present work studies hydrogels’ viability, morphology, and composition based on alginate and gelatin crosslinked with CaCl2 and genipin. The hydrogels were synthesized based on natural polymers such as sodium alginate and gelatin using ionic and natural crosslinking methods. The samples were characterized using ATR-FTIR, SEM, and MTT assay for cell viability. The obtained results revealed that the Caco-2 and HUVEC cell lines with which a tissue model can be developed to evaluate colorectal cancer drugs showed an improvement of the results in the gelatin, alginate, and CaCl2 hydrogels (68.92%) and the gelatin, alginate hydrogels with genipin at 0.25% w/v (60% in HUVEC and 75.07% in Caco-2). The results obtained demonstrate the potential use of alginate/gelatin bioinks for 3D human tissue models.

• Hoofdauteur: Bonilla Vázquez, Alicia del Rocío (author)
• Formaat: bachelorThesis
• Taal: eng
• Gepubliceerd in: 2023
• Onderwerpen: Biotintas; Hidrogeles; Bioimpresión 3D; Bioink; Hydrogels; 3D bioprinting
• Online toegang: http://repositorio.yachaytech.edu.ec/handle/123456789/692