Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models
Currently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, t...
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Formatua: | bachelorThesis |
Hizkuntza: | eng |
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2023
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Sarrera elektronikoa: | http://repositorio.yachaytech.edu.ec/handle/123456789/692 |
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author | Bonilla Vázquez, Alicia del Rocío |
author_facet | Bonilla Vázquez, Alicia del Rocío |
author_role | author |
collection | Repositorio Universidad Yachay Tech |
dc.contributor.none.fl_str_mv | Briceño Araujo, Sarah Elisa Spencer Valero, Lilian Maritza |
dc.creator.none.fl_str_mv | Bonilla Vázquez, Alicia del Rocío |
dc.date.none.fl_str_mv | 2023-12-07T10:04:20Z 2023-12-07T10:04:20Z 2023-12 |
dc.format.none.fl_str_mv | application/pdf |
dc.identifier.none.fl_str_mv | http://repositorio.yachaytech.edu.ec/handle/123456789/692 |
dc.language.none.fl_str_mv | eng |
dc.publisher.none.fl_str_mv | Universidad de Investigación de Tecnología Experimental Yachay |
dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
dc.source.none.fl_str_mv | reponame:Repositorio Universidad Yachay Tech instname:Universidad Yachay Tech instacron:Yachay |
dc.subject.none.fl_str_mv | Biotintas Hidrogeles Bioimpresión 3D Bioink Hydrogels 3D bioprinting |
dc.title.none.fl_str_mv | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
description | Currently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, these models can be generated that help recreate tumor environments, allowing the behavior of drugs to be studied. Therefore, the aim is to synthesize bioinks with characteristics such as biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and mechanical integrity. The present work studies hydrogels’ viability, morphology, and composition based on alginate and gelatin crosslinked with CaCl2 and genipin. The hydrogels were synthesized based on natural polymers such as sodium alginate and gelatin using ionic and natural crosslinking methods. The samples were characterized using ATR-FTIR, SEM, and MTT assay for cell viability. The obtained results revealed that the Caco-2 and HUVEC cell lines with which a tissue model can be developed to evaluate colorectal cancer drugs showed an improvement of the results in the gelatin, alginate, and CaCl2 hydrogels (68.92%) and the gelatin, alginate hydrogels with genipin at 0.25% w/v (60% in HUVEC and 75.07% in Caco-2). The results obtained demonstrate the potential use of alginate/gelatin bioinks for 3D human tissue models. |
eu_rights_str_mv | openAccess |
format | bachelorThesis |
id | Yachay_2661f2d91c91acee956b1d66ef033b00 |
instacron_str | Yachay |
institution | Yachay |
instname_str | Universidad Yachay Tech |
language | eng |
network_acronym_str | Yachay |
network_name_str | Repositorio Universidad Yachay Tech |
oai_identifier_str | oai:repositorio.yachaytech.edu.ec:123456789/692 |
publishDate | 2023 |
publisher.none.fl_str_mv | Universidad de Investigación de Tecnología Experimental Yachay |
reponame_str | Repositorio Universidad Yachay Tech |
repository.mail.fl_str_mv | . |
repository.name.fl_str_mv | Repositorio Universidad Yachay Tech - Universidad Yachay Tech |
repository_id_str | 10284 |
spelling | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue modelsBonilla Vázquez, Alicia del RocíoBiotintasHidrogelesBioimpresión 3DBioinkHydrogels3D bioprintingCurrently, the likelihood of a drug in cancer treatments passing clinical trials is low because they are tested in animal models or 2D cell cultures, which do not provide a precise system. Researchers have seen 3D tissue models as an alternative for oncological research since, with 3D bioprinting, these models can be generated that help recreate tumor environments, allowing the behavior of drugs to be studied. Therefore, the aim is to synthesize bioinks with characteristics such as biocompatibility, biodegradability, non-toxicity, non-immunogenicity, and mechanical integrity. The present work studies hydrogels’ viability, morphology, and composition based on alginate and gelatin crosslinked with CaCl2 and genipin. The hydrogels were synthesized based on natural polymers such as sodium alginate and gelatin using ionic and natural crosslinking methods. The samples were characterized using ATR-FTIR, SEM, and MTT assay for cell viability. The obtained results revealed that the Caco-2 and HUVEC cell lines with which a tissue model can be developed to evaluate colorectal cancer drugs showed an improvement of the results in the gelatin, alginate, and CaCl2 hydrogels (68.92%) and the gelatin, alginate hydrogels with genipin at 0.25% w/v (60% in HUVEC and 75.07% in Caco-2). The results obtained demonstrate the potential use of alginate/gelatin bioinks for 3D human tissue models.Actualmente, la probabilidad de que un fármaco en tratamientos contra el cáncer pase los ensayos clínicos es baja porque se prueban en modelos animales o cultivos celulares 2D, que no proporcionan un sistema preciso. Los investigadores han visto los modelos de tejidos 3D como una alternativa para la investigación oncológica ya que, con la bioimpresión 3D, se pueden generar estos modelos que ayudan a recrear entornos tumorales, permitiendo estudiar el comportamiento de los fármacos. Por tanto, el objetivo es sintetizar biotintas con características como biocompatibilidad, biodegradabilidad, no toxicidad, no inmunogenicidad e integridad mecánica. El presente trabajo estudia la viabilidad, morfología y composición de hidrogeles basados en alginato y gelatina entrecruzados con CaCl2 y genipina. Los hidrogeles se sintetizaron a partir de polímeros naturales como el alginato de sodio y la gelatina utilizando métodos de reticulación iónicos y naturales. Las muestras se caracterizaron mediante ensayos ATR-FTIR, SEM y MTT para determinar la viabilidad celular. Los resultados obtenidos revelaron que las líneas celulares Caco-2 y HUVEC con las que se puede desarrollar un modelo de tejido para evaluar fármacos contra el cáncer colorrectal mostraron una mejora de los resultados en los hidrogeles de gelatina, alginato y CaCl2 (68,92%) y en la gelatina, alginato. hidrogeles con genipina al 0,25% p/v (60% en HUVEC y 75,07% en Caco-2). Los resultados obtenidos demuestran el uso potencial de biotintas de alginato/gelatina para modelos 3D de tejido humano.Ingeniero/a Biomédico/aUniversidad de Investigación de Tecnología Experimental YachayBriceño Araujo, Sarah ElisaSpencer Valero, Lilian Maritza2023-12-07T10:04:20Z2023-12-07T10:04:20Z2023-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://repositorio.yachaytech.edu.ec/handle/123456789/692enginfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Yachay Techinstname:Universidad Yachay Techinstacron:Yachay2025-07-08T17:48:37Zoai:repositorio.yachaytech.edu.ec:123456789/692Institucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oaiEcuador...opendoar:102842025-07-08T17:48:37falseInstitucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oai.Ecuador...opendoar:102842025-07-08T17:48:37Repositorio Universidad Yachay Tech - Universidad Yachay Techfalse |
spellingShingle | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models Bonilla Vázquez, Alicia del Rocío Biotintas Hidrogeles Bioimpresión 3D Bioink Hydrogels 3D bioprinting |
status_str | publishedVersion |
title | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
title_full | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
title_fullStr | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
title_full_unstemmed | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
title_short | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
title_sort | Synthesis and characterization of alignate/gelatin bioinks for 3d human tissue models |
topic | Biotintas Hidrogeles Bioimpresión 3D Bioink Hydrogels 3D bioprinting |
url | http://repositorio.yachaytech.edu.ec/handle/123456789/692 |