Validation of a methodology by LC-MS/MS for the determination of carbamate pesticide residues in organic ecuadorian bananas

The production and export of bananas in Ecuador have become crucial in its economy, positioning the country as a leading worldwide player in the banana industry. This relevance has generated the need to focus research on plant safety to guarantee safe levels of pesticide residues. In this study, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method version EN 15662 was validated for the multi-residual analysis of four carbamates in organic banana samples. This method was applied in the extraction and cleaning stages of the banana samples, which were then analyzed by liquid chromatography coupled to a triple quadrupole mass spectrometer (LC-MS/MS) with electrospray ionization (ESI). During the validation process, evaluations of several parameters were performed in terms of absolute and relative quantification. These procedures covered the evaluation of the matrix effect, the verification of linearity, the determination of precision and accuracy, the establishment of the limits of detection (LOD) and quantitation (LOQ), and the confirmation of specificity and selectivity of the method. Likewise, the calculation of the expanded uncertainty of the analytical procedure was carried out. Quantifying using recovery standards in relative terms yielded satisfactory results. In this sense, the method demonstrated recoveries that were in a range of 76% to 118%, with coefficients of variation (CV) ≤ 20%. The limits of detection and quantification were set in a range of 1.49 to 2.57 μg/kg and 2.99 to 5.067 μ/kg, respectively. In relation to linearity, determination coefficients r2 equal to or greater than 0.997 were achieved, and residuals remained ± 20%. The linearity range began from concentrations of 0.010 mg/L for propamocarb and carbofuran and 0.005 and 0.002 mg/L for methiocarb and methomyl, respectively. Finally, the expanded uncertainty of the method was estimated to be 48.26%.

• Huvudupphovsman: Carapaz Cuasapaz, Daniela Pastora (author)
• Materialtyp: bachelorThesis
• Språk: eng
• Publicerad: 2024
• Ämnen: Plaguicidas; Validación de métodos; Carbamatos; Pesticides; Method validation; Carbamates
• Länkar: http://repositorio.yachaytech.edu.ec/handle/123456789/771