Phage display technology: capabilities and applications

Today it is theoretically possible to isolate antibodies to any target using a bacterial virus known as bacteriophage. With this virus, libraries with millions of copies of antibodies are created, through phage display, a specific antibody for the said target is identified. The technique involves the pick up of antibody genes from human donors, the cloning and expression of these said genes on the surface of the phage, and identifying the phage whose sequence expresses an antibody with the necessary properties for the correct interaction with its respective antigen. Once the antibody with the desired properties is identified, its genetic sequence is recovered and multiple copies of it are produced within cultured cells. The relevance of this technique lies in the fact that the antibodies obtained at the end of this process can be used for different treatments against cancer, autoimmune diseases, and viral infections, obviously without neglecting the respective evaluations of their efficiency for approval and use. This work aims to explain the biology behind the steps involved in the antibody isolation by phage display, to understand the scope of this technology, and to review the effect and consequences of antibodies that have already been approved or are in clinical trials to date.

• 主要作者: Merchán Muñoz, Brian David (author)
• 格式: bachelorThesis
• 語言: eng
• 出版: 2022
• 主題: Anticuerpos; Virus; Bacteriófago; Phage display; Sistema inmune; Antibody; Bacteriophage; Immune system
• 在線閱讀: http://repositorio.yachaytech.edu.ec/handle/123456789/502