Estudio de la expresión del gen STR3 de Saccharomyces cerevisiae en presencia de pesticidas comúnmente usados en Ecuador

The modern agricultural industry faces an important dilemma as the global population is rapidly increasing. Consequently, the use of pesticides has intensified to maintain crop production. Fungicides have proven relatively effective in controlling certain pests and diseases in crops; however, due to their low specificity, they have been linked to adverse effects on various ecosystems. This highlights the need for new monitoring tools. The aim of this project is to develop an initial biosensor based on the modification of Saccharomyces cerevisiae for the detection and quantification of pyrimethanil, a fungicide commonly used in Ecuadorian agriculture. To achieve this, the promoter of the STR3 gene was cloned and fused to the GFP reporter gene, which was expressed in S. cerevisiae strains exposed to pyrimethanil. The results show that higher concentrations of the fungicide significantly affected yeast growth, indicating a toxic effect. Fluorescence analysis revealed that the GFP expression system responded to induced stress, with fluorescence intensity correlating to higher concentrations (20 and 110 mg/L), differentiating it from negative controls in each experimental treatment. The 4-hour time interval was the most sensitive, recording the highest intensity. The fluorescence response suggests that the STR3 promoter is relevant for constructing the proposed biosensor, reinforcing its potential as a tool for detecting and monitoring pyrimethanil and other pesticides.

• Main Author: Robles Ruíz, Paulo César (author)
• Format: masterThesis
• Language: spa
• Published: 2024
• Subjects: Funguicidas; Biosensores; Toxicidad
• Online Access: http://repositorio.yachaytech.edu.ec/handle/123456789/882