Biological and chemical analysis of sludge from the wastewater treatment system in a mining industry camp

Chemical and biological analyzes are an essential part of wastewater plants. In a mining camp, we have to consider that the pollution produced by the industry and our waste can represent an environmental problem. Wastewater represents today a socio-environmental problem. However, their treatment can provide us with immediate solutions. In order to check if these wastewater treatment plants are working correctly, we will conduct different tests. This is how we have, to begin with, an analysis of the classification code of the characteristics that hazardous waste contains and what it means: corrosive, reactive, explosive, toxic, flammable, and biologically infectious (CRETIB). With this, we knew if our samples would be considered risky. To start with the tests, we made a pH and dissolved oxygen tests, which work as bioindicators inside the WWTP. Then we moved on to the germination test with the WWTP sludge in Petri dishes and plastic germinators. So, we can check if they show growth inhibition. We will also have bacterial growth as a bioindicator, using serial dilutions in TSB and seeding them in LB agar media. Finally, considering that antibiotic resistance represents one of the biggest problems facing human and animal health. We will have a DNA extraction from the sewage sludge, where the interest was to sequence and find the antibiotic-resistance genes in our samples. With a vast literature, we will be able to identify the resistance genes we expect to find in our samples and the possible solutions.

• Päätekijä: Cobos Granda, Dayana Elizabeth (author)
• Aineistotyyppi: bachelorThesis
• Kieli: eng
• Julkaistu: 2023
• Aiheet: Aguas residuales; Tratamiento de aguas residuales; Wastewater; Wastewater Treatment Plant (WWTP)
• Linkit: http://repositorio.yachaytech.edu.ec/handle/123456789/612