Synthesis of chitosan fibers by electrospinning
Electrospun fibers based on chitosan have been studied for their biocompatibility and antibacterial properties. Nevertheless, producing pure chitosan fibers through electrospinning is challenging due to its rigid chemical structure. This study synthesizes chitosan fibers by optimizing polymer soluti...
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| Формат: | bachelorThesis |
| Мова: | eng |
| Опубліковано: |
2023
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| Предмети: | |
| Онлайн доступ: | http://repositorio.yachaytech.edu.ec/handle/123456789/663 |
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Додати тег | Резюме: | Electrospun fibers based on chitosan have been studied for their biocompatibility and antibacterial properties. Nevertheless, producing pure chitosan fibers through electrospinning is challenging due to its rigid chemical structure. This study synthesizes chitosan fibers by optimizing polymer solution and electrospinning process parameters. Several concentrations of chitosan were produced from shrimp, crab shells, and commercial chitosan(Sigma-Aldrich). The solvents tested were dilute Acetic Acid (AcOH), Trifluoroacetic acid (TFA) 99 % purity, and Dichloromethane (DCM) . To improve their conductivity, some solutions were reinforced with Multi Walled Carbon Nanotubes (MWCNTs). Several chitosan solutions were tested for electrospinning, but none produced a visible jet when the electric field was applied. Only when TFA was used as the solvent, chitosan fibers were deposited onto the collector. The results showed that when the chitosan concentration was increased, the morphology of the deposition on the collector changed from spherical beads to interconnected fiber. The homogeneity of the electrospun chitosan fiber was improved by adding DCM to the chitosan-TF solution with a volume ratio 70:30 v/v. Scanning electron microscopy (SEM) analyzed the morphology of the fibers; before observation, the samples were coated with gold using plasma sputtering. The samples that provided the best fibers were Sigma-Aldrich chitosan and crab chitosan at a concentration of 4 % (w/v) using TFA/DCM as solvent. Upon subjecting the fibers to chemical analysis via Raman spectroscopy, the results indicated the presence of amino groups (NH_2) that are characteristic of chitosan. It is interesting to note that the solvent-related peaks (CF_3) were not visibly prominent in the spectra, indicating that the electrospinning process was successful in eliminating all the solvent. The successful production of chitosan fibers offers a new approach to creating smart scaffolds for tissue engineering. |
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