Phytochemical characterization and antibacterial activity of bioactive components from methanolic extracts of Eupatorium glutinosum Lam. leaves
Traditional medicine has been used since prehistoric times. The use of medicinal plants is the principal tool of this type of therapy. Indigenous communities used medical plants owing to their excellent biological response in the human body. Due to the mentioned, the knowledge earned by the indigeno...
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| Materialtyp: | bachelorThesis |
| Språk: | eng |
| Publicerad: |
2021
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| Ämnen: | |
| Länkar: | http://repositorio.yachaytech.edu.ec/handle/123456789/310 |
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Lägg till en tagg | Sammanfattning: | Traditional medicine has been used since prehistoric times. The use of medicinal plants is the principal tool of this type of therapy. Indigenous communities used medical plants owing to their excellent biological response in the human body. Due to the mentioned, the knowledge earned by the indigenous communities in traditional medicine is transmitted through time from generation to generation. Several investigations have demonstrated that the medicinal plants' successful biological response is due to the secondary metabolites. Eupatorium glutinosum Lam, popularly called Matico, is a medicinal plant used in traditional Ecuadorian medicine. In this work, state-of-the-art about Eupatorium glutinosum Lam is reviewed, and the phytochemical study of methanol and aqueous extracts is deepened. Methanolic extracts were prepared using Soxhlet and Maceration procedures, while aqueous extract was obtained by maceration. After identifying the secondary metabolites present in the methanolic extracts, they were subjected to separation by column chromatography. The obtained fractions were analyzed by Thin Layer Chromatography (TLC) and high-resolution liquid chromatography (HPLC), to determine components and purity of the fractions. Besides, infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) were used to establish the nature of the functional groups present in each fraction's compounds. Finally, the antibacterial activity of the extracts and isolated fractions was studied by means of agar diffusion methods and optical density measurements with the bacterial strain Escherichia coli (DH5-α), obtaining promising results, since several of the samples analyzed showed antimicrobial activity similar to that observed for the positive control (Ampicillin). An analysis of all the results allowed us to suggest the nature of the methanolic extracts' biologically active components. The results are not definitive since an exhaustive structural study is needed to elucidate the molecules present in the extracts studied unequivocally. |
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