The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications

Diatoms are an abundant living being along the planet, from which their outer structures are quasi symmetrical and thus represent an ideal template for further functionalization and plasmonic decoration. This study performs an extensive analysis where light wavelength is tested as reductor agent dur...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autor principal: León Valencia, Adrián Javier (author)
Format: bachelorThesis
Idioma:eng
Publicat: 2021
Matèries:
Accés en línia:http://repositorio.yachaytech.edu.ec/handle/123456789/339
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
_version_ 1858461406714658816
author León Valencia, Adrián Javier
author_facet León Valencia, Adrián Javier
author_role author
collection Repositorio Universidad Yachay Tech
dc.contributor.none.fl_str_mv González Vázquez, Gema
Briceño Araujo, Sarah Elisa
dc.creator.none.fl_str_mv León Valencia, Adrián Javier
dc.date.none.fl_str_mv 2021-06-15T19:47:38Z
2021-06-15T19:47:38Z
2021-06
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://repositorio.yachaytech.edu.ec/handle/123456789/339
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Universidad de Investigación de Tecnología Experimental Yachay
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Yachay Tech
instname:Universidad Yachay Tech
instacron:Yachay
dc.subject.none.fl_str_mv Diatomeas
Frústulas
Sensores
Biosensores
Nanopartículas
Efecto de luz
Diatoms
Frustules
Sensors
Biosensors
Nanoparticles
Light effect
dc.title.none.fl_str_mv The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
description Diatoms are an abundant living being along the planet, from which their outer structures are quasi symmetrical and thus represent an ideal template for further functionalization and plasmonic decoration. This study performs an extensive analysis where light wavelength is tested as reductor agent during the in-situ synthesis process of both Gold and Silver nanoparticles. Different exposure times and an additional reductor agent are used to compare the efficiency and effects of nanoparticles sizes and shapes. Finally, comparative analysis were developed before and after DNA attachment, with fluorescence and raman spectroscopy techniques, to decorated frustules in order to test viability as biosensors. From here, Silver decoration seems to work the best for sensing under raman signals, while Gold decoration under fluorescence microscopy.
eu_rights_str_mv openAccess
format bachelorThesis
id Yachay_e1ecd06bbcec33e31ca6eaeff070f2a4
instacron_str Yachay
institution Yachay
instname_str Universidad Yachay Tech
language eng
network_acronym_str Yachay
network_name_str Repositorio Universidad Yachay Tech
oai_identifier_str oai:repositorio.yachaytech.edu.ec:123456789/339
publishDate 2021
publisher.none.fl_str_mv Universidad de Investigación de Tecnología Experimental Yachay
reponame_str Repositorio Universidad Yachay Tech
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Yachay Tech - Universidad Yachay Tech
repository_id_str 10284
spelling The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applicationsLeón Valencia, Adrián JavierDiatomeasFrústulasSensoresBiosensoresNanopartículasEfecto de luzDiatomsFrustulesSensorsBiosensorsNanoparticlesLight effectDiatoms are an abundant living being along the planet, from which their outer structures are quasi symmetrical and thus represent an ideal template for further functionalization and plasmonic decoration. This study performs an extensive analysis where light wavelength is tested as reductor agent during the in-situ synthesis process of both Gold and Silver nanoparticles. Different exposure times and an additional reductor agent are used to compare the efficiency and effects of nanoparticles sizes and shapes. Finally, comparative analysis were developed before and after DNA attachment, with fluorescence and raman spectroscopy techniques, to decorated frustules in order to test viability as biosensors. From here, Silver decoration seems to work the best for sensing under raman signals, while Gold decoration under fluorescence microscopy.Las diatomeas son un ser vivo abundante a lo largo del planeta, de las cuales, sus estructuras externas son quasi-simétricas y representan una base ideal para posterior funcionalización y decoración con nanopartículas plasmónicas. Este estudio realiza un análisis extensivo, donde la longitud de onda de la luz es probada como un agente reductor durante el proceso de síntesis in-situ de nanopartículas de oro y de plata. Diferentes tiempos de exposición y un agente reductor adicional son usados para comparar la eficiencia y efectos de los distintos tamaños y formas de las nanopartículas. Finalmente, análisis comparativos fueron desarrollados antes y después de añadir ADN, con técnicas como fluorescencia y espectroscopia raman, a las frústulas decoradas con el fin de probar su habilidad como biosensores. A partir de estos resultados, decoración con nanopartículas de plata funciona de mejor manera como sensor para señales raman, mientras que decoración con nanopartículas de oro funciona mejor bajo microscopía de fluorescencia.Ingeniero/a en NanotecnologíaUniversidad de Investigación de Tecnología Experimental YachayGonzález Vázquez, GemaBriceño Araujo, Sarah Elisa2021-06-15T19:47:38Z2021-06-15T19:47:38Z2021-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://repositorio.yachaytech.edu.ec/handle/123456789/339enginfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Yachay Techinstname:Universidad Yachay Techinstacron:Yachay2025-07-08T17:51:12Zoai:repositorio.yachaytech.edu.ec:123456789/339Institucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oaiEcuador...opendoar:102842025-07-08T17:51:12falseInstitucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oai.Ecuador...opendoar:102842025-07-08T17:51:12Repositorio Universidad Yachay Tech - Universidad Yachay Techfalse
spellingShingle The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
León Valencia, Adrián Javier
Diatomeas
Frústulas
Sensores
Biosensores
Nanopartículas
Efecto de luz
Diatoms
Frustules
Sensors
Biosensors
Nanoparticles
Light effect
status_str publishedVersion
title The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
title_full The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
title_fullStr The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
title_full_unstemmed The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
title_short The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
title_sort The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
topic Diatomeas
Frústulas
Sensores
Biosensores
Nanopartículas
Efecto de luz
Diatoms
Frustules
Sensors
Biosensors
Nanoparticles
Light effect
url http://repositorio.yachaytech.edu.ec/handle/123456789/339