The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications
Diatoms are an abundant living being along the planet, from which their outer structures are quasi symmetrical and thus represent an ideal template for further functionalization and plasmonic decoration. This study performs an extensive analysis where light wavelength is tested as reductor agent dur...
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| Format: | bachelorThesis |
| Idioma: | eng |
| Publicat: |
2021
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| Accés en línia: | http://repositorio.yachaytech.edu.ec/handle/123456789/339 |
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| _version_ | 1858461406714658816 |
|---|---|
| author | León Valencia, Adrián Javier |
| author_facet | León Valencia, Adrián Javier |
| author_role | author |
| collection | Repositorio Universidad Yachay Tech |
| dc.contributor.none.fl_str_mv | González Vázquez, Gema Briceño Araujo, Sarah Elisa |
| dc.creator.none.fl_str_mv | León Valencia, Adrián Javier |
| dc.date.none.fl_str_mv | 2021-06-15T19:47:38Z 2021-06-15T19:47:38Z 2021-06 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://repositorio.yachaytech.edu.ec/handle/123456789/339 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidad de Investigación de Tecnología Experimental Yachay |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Yachay Tech instname:Universidad Yachay Tech instacron:Yachay |
| dc.subject.none.fl_str_mv | Diatomeas Frústulas Sensores Biosensores Nanopartículas Efecto de luz Diatoms Frustules Sensors Biosensors Nanoparticles Light effect |
| dc.title.none.fl_str_mv | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Diatoms are an abundant living being along the planet, from which their outer structures are quasi symmetrical and thus represent an ideal template for further functionalization and plasmonic decoration. This study performs an extensive analysis where light wavelength is tested as reductor agent during the in-situ synthesis process of both Gold and Silver nanoparticles. Different exposure times and an additional reductor agent are used to compare the efficiency and effects of nanoparticles sizes and shapes. Finally, comparative analysis were developed before and after DNA attachment, with fluorescence and raman spectroscopy techniques, to decorated frustules in order to test viability as biosensors. From here, Silver decoration seems to work the best for sensing under raman signals, while Gold decoration under fluorescence microscopy. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | Yachay_e1ecd06bbcec33e31ca6eaeff070f2a4 |
| instacron_str | Yachay |
| institution | Yachay |
| instname_str | Universidad Yachay Tech |
| language | eng |
| network_acronym_str | Yachay |
| network_name_str | Repositorio Universidad Yachay Tech |
| oai_identifier_str | oai:repositorio.yachaytech.edu.ec:123456789/339 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Universidad de Investigación de Tecnología Experimental Yachay |
| reponame_str | Repositorio Universidad Yachay Tech |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Yachay Tech - Universidad Yachay Tech |
| repository_id_str | 10284 |
| spelling | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applicationsLeón Valencia, Adrián JavierDiatomeasFrústulasSensoresBiosensoresNanopartículasEfecto de luzDiatomsFrustulesSensorsBiosensorsNanoparticlesLight effectDiatoms are an abundant living being along the planet, from which their outer structures are quasi symmetrical and thus represent an ideal template for further functionalization and plasmonic decoration. This study performs an extensive analysis where light wavelength is tested as reductor agent during the in-situ synthesis process of both Gold and Silver nanoparticles. Different exposure times and an additional reductor agent are used to compare the efficiency and effects of nanoparticles sizes and shapes. Finally, comparative analysis were developed before and after DNA attachment, with fluorescence and raman spectroscopy techniques, to decorated frustules in order to test viability as biosensors. From here, Silver decoration seems to work the best for sensing under raman signals, while Gold decoration under fluorescence microscopy.Las diatomeas son un ser vivo abundante a lo largo del planeta, de las cuales, sus estructuras externas son quasi-simétricas y representan una base ideal para posterior funcionalización y decoración con nanopartículas plasmónicas. Este estudio realiza un análisis extensivo, donde la longitud de onda de la luz es probada como un agente reductor durante el proceso de síntesis in-situ de nanopartículas de oro y de plata. Diferentes tiempos de exposición y un agente reductor adicional son usados para comparar la eficiencia y efectos de los distintos tamaños y formas de las nanopartículas. Finalmente, análisis comparativos fueron desarrollados antes y después de añadir ADN, con técnicas como fluorescencia y espectroscopia raman, a las frústulas decoradas con el fin de probar su habilidad como biosensores. A partir de estos resultados, decoración con nanopartículas de plata funciona de mejor manera como sensor para señales raman, mientras que decoración con nanopartículas de oro funciona mejor bajo microscopía de fluorescencia.Ingeniero/a en NanotecnologíaUniversidad de Investigación de Tecnología Experimental YachayGonzález Vázquez, GemaBriceño Araujo, Sarah Elisa2021-06-15T19:47:38Z2021-06-15T19:47:38Z2021-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://repositorio.yachaytech.edu.ec/handle/123456789/339enginfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Yachay Techinstname:Universidad Yachay Techinstacron:Yachay2025-07-08T17:51:12Zoai:repositorio.yachaytech.edu.ec:123456789/339Institucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oaiEcuador...opendoar:102842025-07-08T17:51:12falseInstitucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oai.Ecuador...opendoar:102842025-07-08T17:51:12Repositorio Universidad Yachay Tech - Universidad Yachay Techfalse |
| spellingShingle | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications León Valencia, Adrián Javier Diatomeas Frústulas Sensores Biosensores Nanopartículas Efecto de luz Diatoms Frustules Sensors Biosensors Nanoparticles Light effect |
| status_str | publishedVersion |
| title | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| title_full | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| title_fullStr | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| title_full_unstemmed | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| title_short | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| title_sort | The optical properties of diatoms doped with plasmonic nanoparticles for biomedical applications |
| topic | Diatomeas Frústulas Sensores Biosensores Nanopartículas Efecto de luz Diatoms Frustules Sensors Biosensors Nanoparticles Light effect |
| url | http://repositorio.yachaytech.edu.ec/handle/123456789/339 |