Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time
Agricultural waste management is a growing concern due to the large amounts of organic residues generated, including fruit peels. Dragon Fruit peel (Selenicereus megalanthus) represents a significant by-product with valuable bioactive compounds, including enzymes. This study aimed to evaluate the pr...
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| Format: | bachelorThesis |
| Publicat: |
2025
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| Matèries: | |
| Accés en línia: | https://repositorio.yachaytech.edu.ec/handle/123456789/995 |
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Afegir etiqueta | Sumari: | Agricultural waste management is a growing concern due to the large amounts of organic residues generated, including fruit peels. Dragon Fruit peel (Selenicereus megalanthus) represents a significant by-product with valuable bioactive compounds, including enzymes. This study aimed to evaluate the proteolytic capacity of Dragon Fruit peel extract as a function of time, analyzing enzymatic activity on sodium caseinate plate, spectrophotometric methods and kinetic modeling using the Michaelis-Menten equation. Enzyme extracts were obtained using two different buffer-peel ratios of dilution (3:1 and 1:1), with the 1:1 used due to its operational advantage of requiring a smaller buffer volume for preparation. Additionally, kinetic analysis shows that the Dragon Fruit peel enzyme extract exhibited a high affinity for sodium casein substrate, demonstrating the potential for protein hydrolysis. These findings show the possible application of Dragon Fruit peel enzymes in the industry, particularly in waste valorization strategies. |
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