Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time
Agricultural waste management is a growing concern due to the large amounts of organic residues generated, including fruit peels. Dragon Fruit peel (Selenicereus megalanthus) represents a significant by-product with valuable bioactive compounds, including enzymes. This study aimed to evaluate the pr...
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| Médium: | bachelorThesis |
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2025
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| On-line přístup: | https://repositorio.yachaytech.edu.ec/handle/123456789/995 |
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Přidat tag | _version_ | 1863534788273504256 |
|---|---|
| author | Erazo Muriel, Ana Liset |
| author_facet | Erazo Muriel, Ana Liset |
| author_role | author |
| collection | Repositorio Universidad Yachay Tech |
| dc.contributor.none.fl_str_mv | Pazmiño Arias, Carlos Esteban Cortez Latorre, Juan Diego |
| dc.creator.none.fl_str_mv | Erazo Muriel, Ana Liset |
| dc.date.none.fl_str_mv | 2025-09-15T19:15:06Z 2025-09 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://repositorio.yachaytech.edu.ec/handle/123456789/995 |
| dc.language.none.fl_str_mv | en_US |
| dc.publisher.none.fl_str_mv | Universidad de Investigación de Tecnología Experimental Yachay |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Yachay Tech instname:Universidad Yachay Tech instacron:Yachay |
| dc.subject.none.fl_str_mv | Espectrofotometría Desechos agrícolas Enzimas proteolíticas Spectrophotometry Food waste Proteolytic enzymes |
| dc.title.none.fl_str_mv | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Agricultural waste management is a growing concern due to the large amounts of organic residues generated, including fruit peels. Dragon Fruit peel (Selenicereus megalanthus) represents a significant by-product with valuable bioactive compounds, including enzymes. This study aimed to evaluate the proteolytic capacity of Dragon Fruit peel extract as a function of time, analyzing enzymatic activity on sodium caseinate plate, spectrophotometric methods and kinetic modeling using the Michaelis-Menten equation. Enzyme extracts were obtained using two different buffer-peel ratios of dilution (3:1 and 1:1), with the 1:1 used due to its operational advantage of requiring a smaller buffer volume for preparation. Additionally, kinetic analysis shows that the Dragon Fruit peel enzyme extract exhibited a high affinity for sodium casein substrate, demonstrating the potential for protein hydrolysis. These findings show the possible application of Dragon Fruit peel enzymes in the industry, particularly in waste valorization strategies. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | Yachay_f7df4147fd493faf6bcccfc03ddf3d8b |
| instacron_str | Yachay |
| institution | Yachay |
| instname_str | Universidad Yachay Tech |
| language_invalid_str_mv | en_US |
| network_acronym_str | Yachay |
| network_name_str | Repositorio Universidad Yachay Tech |
| oai_identifier_str | oai:repositorio.yachaytech.edu.ec:123456789/995 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Universidad de Investigación de Tecnología Experimental Yachay |
| reponame_str | Repositorio Universidad Yachay Tech |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Yachay Tech - Universidad Yachay Tech |
| repository_id_str | 10284 |
| spelling | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of timeErazo Muriel, Ana LisetEspectrofotometríaDesechos agrícolasEnzimas proteolíticasSpectrophotometryFood wasteProteolytic enzymesAgricultural waste management is a growing concern due to the large amounts of organic residues generated, including fruit peels. Dragon Fruit peel (Selenicereus megalanthus) represents a significant by-product with valuable bioactive compounds, including enzymes. This study aimed to evaluate the proteolytic capacity of Dragon Fruit peel extract as a function of time, analyzing enzymatic activity on sodium caseinate plate, spectrophotometric methods and kinetic modeling using the Michaelis-Menten equation. Enzyme extracts were obtained using two different buffer-peel ratios of dilution (3:1 and 1:1), with the 1:1 used due to its operational advantage of requiring a smaller buffer volume for preparation. Additionally, kinetic analysis shows that the Dragon Fruit peel enzyme extract exhibited a high affinity for sodium casein substrate, demonstrating the potential for protein hydrolysis. These findings show the possible application of Dragon Fruit peel enzymes in the industry, particularly in waste valorization strategies.La gestión de residuos agrícolas se ha convertido en una preocupación creciente debido a la gran cantidad de desechos orgánicos que se generan, especialmente las cáscaras de frutas. La cáscara de pitahaya (Selenicereus megalanthus) es un subproducto importante que contiene compuestos bioactivos valiosos, entre ellos enzimas. El objetivo de este trabajo fue evaluar la capacidad de proteólisis en el extracto de cáscara de pitahaya a largo del tiempo, mediante actividad enzimática utilizando métodos como la espectrofotometría y el plato de caseinato de sodio, también se usó la ecuación de Michaelis-Menten para ver la cinética enzimática. Los extractos enzimáticos se obtuvieron utilizando dos proporciones diferentes de buffer-cáscara (3:1 y 1:1), siendo el 1:1 el utilizado debido a su ventaja operativa al requerir un menor volumen de buffer para su preparación. Además, el análisis cinético muestra que la cáscara de la Pitahaya tiene una alta afinidad con el substrato de caseinato de sodio, lo que demuestra su potencial para la hidrólisis de proteínas. Estos resultados demuestran una posible aplicación de las enzimas de la cáscara de Pitahaya en la industria, especialmente en estrategias de valorización de residuos.Ingeniero/a en Agroindustria AlimentariaUniversidad de Investigación de Tecnología Experimental YachayPazmiño Arias, Carlos EstebanCortez Latorre, Juan Diego2025-09-15T19:15:06Z2025-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://repositorio.yachaytech.edu.ec/handle/123456789/995en_USinfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Yachay Techinstname:Universidad Yachay Techinstacron:Yachay2026-02-19T05:00:00Zoai:repositorio.yachaytech.edu.ec:123456789/995Institucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oaiEcuador...opendoar:102842026-02-19T05:00falseInstitucionalhttps://repositorio.yachaytech.edu.ec/Universidad públicahttps://www.yachaytech.edu.ec/https://repositorio.yachaytech.edu.ec/oai.Ecuador...opendoar:102842026-02-19T05:00Repositorio Universidad Yachay Tech - Universidad Yachay Techfalse |
| spellingShingle | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time Erazo Muriel, Ana Liset Espectrofotometría Desechos agrícolas Enzimas proteolíticas Spectrophotometry Food waste Proteolytic enzymes |
| status_str | publishedVersion |
| title | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| title_full | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| title_fullStr | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| title_full_unstemmed | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| title_short | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| title_sort | Evaluation of the proteolytic capacity of pitahaya peel extract as a function of time |
| topic | Espectrofotometría Desechos agrícolas Enzimas proteolíticas Spectrophotometry Food waste Proteolytic enzymes |
| url | https://repositorio.yachaytech.edu.ec/handle/123456789/995 |