Design and development of an analytical method using HPLC for the determination of the chemical marker present in Tropaeolum tuberosum

Despite the unquestionable ancestral belief that numerous herbal plants have healing power, the chemical complexity has hampered scientific studies that support their therapeutic activity. In recent years, the pharmaceutical industry, through modern characterization techniques, has focused on developing methods that allow studying these plants in further detail, to launch high-quality products onto the market. The inexistence of an analytical method focused on quality control of Tropaeolum tuberosum (mashua) powder as a raw material has developed the Ecuadorian pharmaceutical industry's interest in researching this endemic Andean medicinal plant. In this way, mashua was the medicinal plant that is subject of study in this project, which was selected due to its well-known anti-inflammatory activity in popular medicine. This research is focused on determining a chemical marker present in mashua, which will be used as a reference pattern in quality control of raw material (mashua powder). The chemical marker was obtained through the design and development of an analytical method based on Reverse Phase High-Performance Liquid Chromatography with UV-Vis detection (RP-HPLC-UV/Vis). The separation of these compounds on the equipment was achieved using both the THERMO L1 (C18) column (150 mm x 4.6 mm and 5 um) and a gradient elution system. The mobile phase consisted of buffer solution pH 2.5 with water ( TEA 0.01 M, H3PO4) as Solvent A, Methanol as Solvent B, and Acetonitrile as Solvent C during 25 minutes. The HPLC method developed in this work demonstrated that a (+)-catechin and quercetin are not appropriate chemical markers for the characterization and quality control of mashua because these two compounds do not exist or exist in a residual quantity in the extract made from the powder of this Andean medicinal plant. Thus, it is necessary to acquire other standards described in the scientific literature for mashua and available in the market in order to find appropriate chemical markers to characterize and do quality control of this plant. This methodology is extremely important to the Ecuadorian pharmaceutical industry because there is not currently any quality control method for mashua, which prevents its commercialization in the national and international market.

• Hoofdauteur: Lozada Flores, Eva Lissette (author)
• Formaat: bachelorThesis
• Taal: eng
• Gepubliceerd in: 2020
• Onderwerpen: Compuestos fenólicos; Mashua; Medicina tradicional; Marcador químico; Fitoquímico cualitativo preliminar; (+)-Catequina; Phenolic compounds; Chemical marker; Preliminary qualitative phytochemical screening; (+)-Catechin
• Online toegang: http://repositorio.yachaytech.edu.ec/handle/123456789/259