Effects of imidacloprid and acetamiprid during the in vitro neuronal differentiation of SH-SY5Y neuroblastoma cells

Neonicotinoids, acetamiprid (ACE) and imidacloprid (IMI), are widely used insecticides in agriculture, and their application has exponentially increased in the recent years. In insects, these compounds act on the central nervous system causing death after a few hours of exposure. Their mode of action is based on the affinity and agonist activity for nicotinic acetylcholine receptors (nAChR). In humans, neonicotinoids were initially thought to have low toxicity due to their poor affinity for the nAChRs of vertebrate organisms. However, recent evidence has revealed neurotoxicity in these organisms. One of the key stages for studying their toxicity is neurodevelopment due to the greater vulnerability to exogenous compounds. The SH-SY5Y cell line was used in this work as an in vitro model for the study of neurodevelopmental toxicity due to its basal expression of functional nAChRs, as well as its differentiation into neuronal-type cells induced by retinoic acid or staurosporine. The SH-SY5Y cell line was exposed to neonicotinoids at three different stages: non-Differentiated cells, On-the-Go-Differentiating cells, and Differentiated cells. (...)

• מחבר ראשי: Guzmán Vallejos, Marcelo Steven (author)
• פורמט: bachelorThesis
• שפה: eng
• יצא לאור: 2022
• נושאים: SH-SY5Y; Imidacloprid; Acetamiprid; Nicotina; Acetilcolina; Neurotoxicidad; Receptor nicotínico de acetilcolina; Neurodesarrollo; Nicotine; Acetylcholine; Nicotinic acetylcholine receptor; Neurodevelopment
• גישה מקוונת: http://repositorio.yachaytech.edu.ec/handle/123456789/568