Actividad probiótica del cóctel microbiano (Lactobacillus plantarum y acidophilus, Bacillus subtilis, Saccharomyces cerevisiae, Chlorella vulgaris) en sanidad y producción del camarón (Litopenaeus vannamei)
The present research work aimed to evaluate the probiotic activity of a compound microbial cocktail (Lactobacillus plantarum 1.70x109 CFU/ml and acidophilus 1.85x109 CFU/ml, Bacillus subtilis 7.80x107 CFU/ml, Saccharomyces cerevisiae 4.32x108 CFU/ml, Chlorella vulgaris 200 cel/ml) in shrimp health a...
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| Format: | bachelorThesis |
| Language: | spa |
| Published: |
2021
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| Online Access: | http://repositorio.espam.edu.ec/handle/42000/1615 |
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Add Tag | Summary: | The present research work aimed to evaluate the probiotic activity of a compound microbial cocktail (Lactobacillus plantarum 1.70x109 CFU/ml and acidophilus 1.85x109 CFU/ml, Bacillus subtilis 7.80x107 CFU/ml, Saccharomyces cerevisiae 4.32x108 CFU/ml, Chlorella vulgaris 200 cel/ml) in shrimp health and production (Litopenaeus vannamei). The experimental unit consisted of two groups, each with 10,000 nauplii: control group and group 1 (microbial cocktail) with an application dose of 1.5ml / dm3 and at the laboratory level of 1.5ml per liter of water. Once the microorganisms were activated, the mixture of the mother culture was made, adding 1 liter of cocktail of microorganisms, 15 liters of water, 1 liter of molasses in a 20-liter tank, it was hermetically closed and in an anaerobic medium, it was allowed to ferment by 9 days. The data obtained from the study groups were statistically analyzed through a student's T test with independent samples using SPSS version 21 IBM statistical software (free). The variables studied were weight gain, length, feed conversion, survival percentage and abiotic parameters of the water, obtaining the following results: control group dissolved oxygen (DO) 4.48mg / l, temperature in ° C 29.2 and the pH 8.07 and for group 1 (microbial cocktail) OD 4.79mg / l, temperature in ° C 29.7 and pH 8.27. Regarding the microbiological analysis for the determination of total and fecal coliforms of the study groups, they revealed positive results for the control group with the presence of total coliforms MPN> 16; and a negative result for group 1 (Microbial cocktail). The application of the microbial cocktail in the culture of white shrimp (Litopenaeus vannamei) proved to be feasible because it generated a weight gain of 1.45 grams in the shrimp and a survival of 65.19%; it also maintained the ideal pH of the water and improved its turbidity. |
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