Microbiota bacteriana en fondo de laguna camaronera del humedal La Segua

Shrimp farming is one of the fastest growing activities in Ecuador and in the world, thus motivating the development of this descriptive research. The influence of the bacterial microbiota present in the bottom of the shrimp lagoon of the La Segua Wetland was evaluated. The research techniques applied were the interview, observation; collection of background samples and molecular identification techniques. For taking samples, 5 quadrants of 100 m2 were established and distributed with the English flag method, for a total of 25 samples, which were transported in cold conditions to the Molecular Biology Laboratory of ESPAM. For the extraction of DNA from the bacteria present in the sediment, the Powersoil ® kit (Qiagen) was used. In the identification and quantification of bacterial strains by means of qPCR, which was worked with the 16SrDNA gene using universal primers 16SrDNA27F and 16SrDNA1492R. The organic matter content was obtained in the bottom laboratory of the Pichilingue Station of INIAP by the calcination method. Evidence of the presence of the Bacillus, Exiguobacterium, Acinetobacter, Prolinoborus, Arthrobacter Planococcus genera with more than 99% homology for all cases, with greater abundance for the Bacillus genus strains. In the sequencing carried out, it was possible to verify that there is microbial activity in the bottom and a content of organic matter with a range of 2.20% allowed for this activity.

• Main Author: Zambrano Cedeño, Ider Josué (author)
• Format: bachelorThesis
• Language: spa
• Published: 2021
• Subjects: Análisis molecular; Sedimiento; Materia orgánica
• Online Access: http://repositorio.espam.edu.ec/handle/42000/1431