Novel Kazal-type proteinase inhibitors from the skin secretion of the Splendid leaf frog, Cruziohyla calcarifer

Peptidase inhibitors have an important role controlling a variety of biological processes. Here, we employed a peptidomic approach including molecular cloning, tandem mass spectrometry and enzymatic assays to reveal 7 Kazal-type proteinase inhibitors (CCKPs) (18 variants) in the skin secretion of the unexplored frog, Cruziohyla calcarifer. All 18 proteins shared the Kazal pattern C-X(7)-C-X(6,7)-C-X(6,7)-Y-X(3)-C-X(2)-C-X(15-21)-C and 3 disulphide bridges. Based on structural comparative analysis, we deemed trypsin and chymotrypsin inhibitory activity in CCKP-1, 4 and CCKP 2, 5, 7, respectively. These peptidase inhibitors presumably play a role to control the balance between other functional peptides produced in the amphibian skin secretions.

• Váldodahkki: Proaño Bolaños, Carolina (author)
• Eará dahkkit: Li, Renjie (author), Zhou, Mei (author), Wang, Lei (author), Xi, Xinping (author), Tapia, Elicio E. (author), Coloma, Luis A. (author), Chen, Tianbao (author), Shaw, Chris (author)
• Materiálatiipa: article
• Almmustuhtton: 2017
• Fáttát: Kazal-type proteinase inhibitors; Frog skin secretion; Peptidomic; Molecular cloning; Tandem mass spectrometry; Cruziohyla calcarifer
• Liŋkkat: https://doi.org/10.1016/j.euprot.2017.02.001; http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/130