Evaluación in vitro del control biológico de oidio (Uncinula necátor) mediante la aplicación de Trichoderma spp aislada del cultivo de Vid (Vitis vinífera).
Globally, the foods that are grown are increasingly harmful to human health, mainly due to the high presence of chemical residues. One of the fundamental problems lies in the excessive and inappropriate use of agricultural inputs to combat fungal diseases in grapevine crops. This is why one of the a...
Wedi'i Gadw mewn:
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| Fformat: | bachelorThesis |
| Cyhoeddwyd: |
2024
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| Pynciau: | |
| Mynediad Ar-lein: | https://repositorio.puce.edu.ec/handle/123456789/42832 |
| Tagiau: |
Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
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Ychwanegu Tag | Crynodeb: | Globally, the foods that are grown are increasingly harmful to human health, mainly due to the high presence of chemical residues. One of the fundamental problems lies in the excessive and inappropriate use of agricultural inputs to combat fungal diseases in grapevine crops. This is why one of the alternatives to reduce the use of chemical fungicides, to manage powdery mildew, is biological control that reduces chemical residues in the fruit. Varieties belonging to the genus Trichoderma are recognized as some of the most used in biological control functions, playing in some situations an antagonistic role against phytopathogenic fungi and bacteria. The main objective of this research was the in vitro evaluation of Trichoderma spp. isolated against powdery mildew in grape cultivation. The research was carried out in the laboratories of the PUCE-I, canton Ibarra, province of Imbabura where it was used in a completely randomized design with two doses of Trichoderma spp (1x103 ufc/ml and 1x105 UFC/ml) and a control as a chemical controller (Myclobutanil), with three repetitions in each treatment. The dual culture technique was used to determine the best dose, obtaining better results in controlling the pathogen with the 1x105ml dose. The humid chamber technique was also carried out where vine leaves were inoculated with the isolated pathogenic fungus and with Trichoderma spp. The respective measurements of diametral growth were carried out after 15 days, where it was evident that with the dose of 1x105 UFC/ml of Trichoderma spp, better control of the pathogenic fungus was obtained. Also as a complementary study, the counting of powdery mildew cells was carried out for a period of 10 hours using the spectrophotometry technique and the dose evaluations were carried out in the field to verify the validity of the research. |
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