Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products
As a requirement to evaluate the quality of food products, the counting of aerobic mesophilic microorganism is used. Effectiveness of applying a fast methodology in order to detect the existence of these microorganisms is crucial to take corrective actions in processing facilities. This study aimed...
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2018
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| author | Intriago Bermúdez, Dariel |
| author2 | Zambrano Alcivar, Marco Antonio Bolaños Lucas, María Isabel Sacón Vera, Ely Fernando |
| author2_role | author author author |
| author_facet | Intriago Bermúdez, Dariel Zambrano Alcivar, Marco Antonio Bolaños Lucas, María Isabel Sacón Vera, Ely Fernando |
| author_role | author |
| collection | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| dc.creator.none.fl_str_mv | Intriago Bermúdez, Dariel Zambrano Alcivar, Marco Antonio Bolaños Lucas, María Isabel Sacón Vera, Ely Fernando |
| dc.date.none.fl_str_mv | 2018-12-20 |
| dc.format.none.fl_str_mv | application/pdf text/html application/zip |
| dc.identifier.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| dc.relation.none.fl_str_mv | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161/169 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161/625 https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161/626 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | Revista ESPAMCIENCIA; Vol. 9 Núm. 2 (2018): REVISTA ESPAMCIENCIA 2018; 114-118 1390-8103 reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabí instname:Escuela Superior Politécnica Agropecuaria de Manabí instacron:ESPAM |
| dc.subject.none.fl_str_mv | Microbiología de alimentos contaminación biológica inóculo recuperación Food microbiology biological contamination inoculum recovery |
| dc.title.none.fl_str_mv | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products Comparación de métodos 3M-Petrifilm TM y convencional para conteo rápido de aerobios en productos de cacao |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares |
| description | As a requirement to evaluate the quality of food products, the counting of aerobic mesophilic microorganism is used. Effectiveness of applying a fast methodology in order to detect the existence of these microorganisms is crucial to take corrective actions in processing facilities. This study aimed to compare the alternative method 2015.13 AOAC vs the traditional method based on tryptone soy agar for aerobes fast counting on chocolate coatings and cocoa powder. The matrix chocolate coating was internally inoculated with Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. The matrix cocoa powder showed natural contamination. 5 levels of inoculum were tested: 60, 380, 700, 36 000, 40 000 UFC/g. The culture temperature was about 35°±1°C during 24±2 hours for the method AOAC 2015.13 and 48±2 hours for the conventional method. Results demonstrated that there are no statistical difference between matrixes based on chocolate coatings and cocoa powder, on 3M-PetrifilmTM and tryptone soy agar plates, for aerobes fast counting with inocula 60 (p=0,506), 380 (p=0,843), 700 (p=0,378), 36 000 (p=0,180) and 40 000 (p=0,180), with a correlation coefficient of r=0.99 (p≤0.05), precision Rp%≤2.0*RDS%Poison and recovery ≥ 80%. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | RevESPAM_6c2b8f23b9c8d88d68720d3fb80008b5 |
| instacron_str | ESPAM |
| institution | ESPAM |
| instname_str | Escuela Superior Politécnica Agropecuaria de Manabí |
| language | spa |
| network_acronym_str | RevESPAM |
| network_name_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| oai_identifier_str | oai:ojs.pkp.sfu.ca:article/161 |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López |
| reponame_str | Revistas Escuela Superior Politécnica Agropecuaria de Manabí |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabí |
| repository_id_str | 0 |
| spelling | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa productsComparación de métodos 3M-Petrifilm TM y convencional para conteo rápido de aerobios en productos de cacaoIntriago Bermúdez, DarielZambrano Alcivar, Marco AntonioBolaños Lucas, María IsabelSacón Vera, Ely FernandoMicrobiología de alimentoscontaminación biológicainóculorecuperaciónFood microbiologybiological contaminationinoculumrecovery As a requirement to evaluate the quality of food products, the counting of aerobic mesophilic microorganism is used. Effectiveness of applying a fast methodology in order to detect the existence of these microorganisms is crucial to take corrective actions in processing facilities. This study aimed to compare the alternative method 2015.13 AOAC vs the traditional method based on tryptone soy agar for aerobes fast counting on chocolate coatings and cocoa powder. The matrix chocolate coating was internally inoculated with Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. The matrix cocoa powder showed natural contamination. 5 levels of inoculum were tested: 60, 380, 700, 36 000, 40 000 UFC/g. The culture temperature was about 35°±1°C during 24±2 hours for the method AOAC 2015.13 and 48±2 hours for the conventional method. Results demonstrated that there are no statistical difference between matrixes based on chocolate coatings and cocoa powder, on 3M-PetrifilmTM and tryptone soy agar plates, for aerobes fast counting with inocula 60 (p=0,506), 380 (p=0,843), 700 (p=0,378), 36 000 (p=0,180) and 40 000 (p=0,180), with a correlation coefficient of r=0.99 (p≤0.05), precision Rp%≤2.0*RDS%Poison and recovery ≥ 80%. Para evaluar la calidad de productos alimenticios se emplea como requisito de cumplimiento el recuento de aerobios mesófilos. La efectividad de aplicar una metodología rápida para detectar la presencia de estos microorganismos es fundamental para la toma de acciones correctivas en plantas procesadoras. El objetivo del estudio fue comparar el método alternativo 2015.13 AOAC con el método tradicional a base de agar triptona de soya para el conteo rápido de aerobios en coberturas de chocolate y polvo de cacao. La matriz coberturas de chocolate fue inoculada internamente con Escherichia coli ATCC 25922 y Staphylococcus aureus ATCC 25923. La matriz polvo de cacao presentó contaminación natural. Se probaron 5 niveles de inóculo: 60, 380, 700, 36 000, 40 000 UFC/g. La temperatura de cultivo fue 35°±1°C durante 24±2 horas para el método AOAC 2015.13 y 48±2 horas para el método convencional. Los resultados evidenciaron que no hay diferencias significativas entre las matrices a base de cobertura de chocolate y polvo de cacao, en las placas 3M-PetrifilmTM y agar triptona de soya, para el recuento rápido de aerobios con los inóculos 60 (p=0,506), 380 (p=0,843), 700 (p=0,378), 36 000 (p=0,180) y 40 000 (p=0,180), con un coeficiente de correlación r=0.99 (p≤0.05), precisión Rp%≤2.0*RDS%Poison y recuperación ≥ 80%.Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López2018-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículo revisado por paresapplication/pdftext/htmlapplication/ziphttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161Revista ESPAMCIENCIA; Vol. 9 Núm. 2 (2018): REVISTA ESPAMCIENCIA 2018; 114-1181390-8103reponame:Revistas Escuela Superior Politécnica Agropecuaria de Manabíinstname:Escuela Superior Politécnica Agropecuaria de Manabíinstacron:ESPAMspahttps://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161/169https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161/625https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161/626info:eu-repo/semantics/openAccess2025-02-25T14:35:35Zoai:ojs.pkp.sfu.ca:article/161Institucionalhttp://revistasespam.espam.edu.ec/Universidad públicahttp://www.espam.edu.ec/http://revistasespam.espam.edu.ec/oai.Ecuador.1390-81031390-597Xopendoar:02025-02-25T14:35:35Revistas Escuela Superior Politécnica Agropecuaria de Manabí - Escuela Superior Politécnica Agropecuaria de Manabífalse |
| spellingShingle | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products Intriago Bermúdez, Dariel Microbiología de alimentos contaminación biológica inóculo recuperación Food microbiology biological contamination inoculum recovery |
| status_str | publishedVersion |
| title | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products |
| title_full | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products |
| title_fullStr | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products |
| title_full_unstemmed | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products |
| title_short | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products |
| title_sort | Comparison of 3M-Petrifilm TM and conventional methods for quick counting of aerobics in cocoa products |
| topic | Microbiología de alimentos contaminación biológica inóculo recuperación Food microbiology biological contamination inoculum recovery |
| url | https://revistasespam.espam.edu.ec/index.php/Revista_ESPAMCIENCIA/article/view/161 |