Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus)
The objective of this research project is to characterize the pectin extracted from the skin of the yellow pitahaya (Hylocereus megalanthus), from the Palora canton of the Procel farm. The analyzes were carried out in the City of Guaranda, Sector Laguacoto II, Laboratories of the Research Department...
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| Үндсэн зохиолч: | |
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| Бусад зохиолчид: | |
| Формат: | bachelorThesis |
| Хэл сонгох: | spa |
| Хэвлэсэн: |
2018
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| Нөхцлүүд: | |
| Онлайн хандалт: | http://dspace.ueb.edu.ec/handle/123456789/2782 |
| Шошгууд: |
Шошго нэмэх
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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| _version_ | 1859198151541391360 |
|---|---|
| author | Águila Vera, Paúl Clemente |
| author2 | Melendes Borja, María Augusta |
| author2_role | author |
| author_facet | Águila Vera, Paúl Clemente Melendes Borja, María Augusta |
| author_role | author |
| collection | Repositorio Universidad Estatal de Bolivar |
| dc.contributor.none.fl_str_mv | Altuna, josé Luis |
| dc.creator.none.fl_str_mv | Águila Vera, Paúl Clemente Melendes Borja, María Augusta |
| dc.date.none.fl_str_mv | 2018-11-06T17:14:35Z 2018-11-06T17:14:35Z 2018 |
| dc.format.none.fl_str_mv | application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| dc.identifier.none.fl_str_mv | http://dspace.ueb.edu.ec/handle/123456789/2782 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Universidad Estatal de Bolívar . Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente . Escuela Ingeniería Agroindustrial |
| dc.relation.none.fl_str_mv | FCCAG.IAI;111 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Estatal de Bolivar instname:Universidad Estatal de Bolivar instacron:UEB |
| dc.subject.none.fl_str_mv | PECTINA HIDROFÍLICOS ESTERIFICACIÓN NEUTRALIZACIÓN |
| dc.title.none.fl_str_mv | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | The objective of this research project is to characterize the pectin extracted from the skin of the yellow pitahaya (Hylocereus megalanthus), from the Palora canton of the Procel farm. The analyzes were carried out in the City of Guaranda, Sector Laguacoto II, Laboratories of the Research Department of the State University of Bolívar, Agroindustrial Engineering Career. After the physical analyzes of the pectins obtained from yellow pitahaya in different stages of maturity and type of acid used in this study, the pH values of both the pectin obtained from T1 (Pitahaya green + citric acid) and T2 (green + acid) hydrochloric) were quite similar from 3.3 to 3.4 respectively, in the same way as for T3 (green pitahaya + citric acid) and T4 (ripe pitahaya + hydrochloric acid) were similar from 4.6 to 4.9 respectively. In the content of humidity and ash, a percentage of 9.6 and 5.4 was obtained regarding T1; of 8.4 and 5.0 for T2; 9.2 and 5.4 for T3: 9.7 and 5.7 regarding T4. In the chemical analyzes, the percentage of the degree of esterification of the pectin obtained from (T1) was 44.4% which indicates that it is a low methoxyl pectin, while the pectins obtained from the treatments (T2, T3, and T4) the percentage of esterification was more than 50.7% which indicates that these pectins are high methoxyl. The protein content of each of the treatments was; T1 (4.7%), T2 (7.1%), T3 (5.8%), T4 (5.2%) respectively, when determining the percentage of Galacturonic anhydride acid (% AAG) which allows to know the degree of purity of the pectic substance; the results were similar for each of the 35% purity treatments of the pectic substance. The Transmittance analyzes in the pectin of yellow pitahaya skin; Green and mature state clearly shows the presence of functional groups characteristic of a pectin; at the wavelength (λ) of 3279 the band corresponding to the -OH functional group is found, for the yellow green pitahaya skin pectin while the same group is evidenced in the mature pectin at a λ of 3336; for λ 2918 and 2920 is the band corresponding to the aliphatic C-H bond in both pectins; in the λ of 1734 is found the band corresponding to the C = O esterified carbonyl group corresponding to the yellow green pitahaya skin pectin, while the same carbonyl group for mature pectin is found in the λ of 1731; in the λ of 1615 and 1629 is the band corresponding to the group of total carbonyls in each of the pectins respectively and in a λ of 1014 and 1015 is the band corresponding to the vibration of the -CO bond of each of the pectins. With all these results, it is concluded that the pitahaya skin pectin in green state plus the use of hydrochloric acid, is the best according to the analyzes performed, adjusting to all the established parameters, it is also the first time that pectin is extracted from the yellow pitahaya eco-type called "Palora" which is a pitahaya endemic to the Ecuadorian East, which is cultivated in the Palora canton, Morona Santiago province |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UEB_4fa8b7bf757dfaa5eaa00c3464bf07a9 |
| instacron_str | UEB |
| institution | UEB |
| instname_str | Universidad Estatal de Bolivar |
| language | spa |
| network_acronym_str | UEB |
| network_name_str | Repositorio Universidad Estatal de Bolivar |
| oai_identifier_str | oai:dspace.ueb.edu.ec:123456789/2782 |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Universidad Estatal de Bolívar . Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente . Escuela Ingeniería Agroindustrial |
| reponame_str | Repositorio Universidad Estatal de Bolivar |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivar |
| repository_id_str | 1735 |
| spelling | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus)Águila Vera, Paúl ClementeMelendes Borja, María AugustaPECTINAHIDROFÍLICOSESTERIFICACIÓNNEUTRALIZACIÓNThe objective of this research project is to characterize the pectin extracted from the skin of the yellow pitahaya (Hylocereus megalanthus), from the Palora canton of the Procel farm. The analyzes were carried out in the City of Guaranda, Sector Laguacoto II, Laboratories of the Research Department of the State University of Bolívar, Agroindustrial Engineering Career. After the physical analyzes of the pectins obtained from yellow pitahaya in different stages of maturity and type of acid used in this study, the pH values of both the pectin obtained from T1 (Pitahaya green + citric acid) and T2 (green + acid) hydrochloric) were quite similar from 3.3 to 3.4 respectively, in the same way as for T3 (green pitahaya + citric acid) and T4 (ripe pitahaya + hydrochloric acid) were similar from 4.6 to 4.9 respectively. In the content of humidity and ash, a percentage of 9.6 and 5.4 was obtained regarding T1; of 8.4 and 5.0 for T2; 9.2 and 5.4 for T3: 9.7 and 5.7 regarding T4. In the chemical analyzes, the percentage of the degree of esterification of the pectin obtained from (T1) was 44.4% which indicates that it is a low methoxyl pectin, while the pectins obtained from the treatments (T2, T3, and T4) the percentage of esterification was more than 50.7% which indicates that these pectins are high methoxyl. The protein content of each of the treatments was; T1 (4.7%), T2 (7.1%), T3 (5.8%), T4 (5.2%) respectively, when determining the percentage of Galacturonic anhydride acid (% AAG) which allows to know the degree of purity of the pectic substance; the results were similar for each of the 35% purity treatments of the pectic substance. The Transmittance analyzes in the pectin of yellow pitahaya skin; Green and mature state clearly shows the presence of functional groups characteristic of a pectin; at the wavelength (λ) of 3279 the band corresponding to the -OH functional group is found, for the yellow green pitahaya skin pectin while the same group is evidenced in the mature pectin at a λ of 3336; for λ 2918 and 2920 is the band corresponding to the aliphatic C-H bond in both pectins; in the λ of 1734 is found the band corresponding to the C = O esterified carbonyl group corresponding to the yellow green pitahaya skin pectin, while the same carbonyl group for mature pectin is found in the λ of 1731; in the λ of 1615 and 1629 is the band corresponding to the group of total carbonyls in each of the pectins respectively and in a λ of 1014 and 1015 is the band corresponding to the vibration of the -CO bond of each of the pectins. With all these results, it is concluded that the pitahaya skin pectin in green state plus the use of hydrochloric acid, is the best according to the analyzes performed, adjusting to all the established parameters, it is also the first time that pectin is extracted from the yellow pitahaya eco-type called "Palora" which is a pitahaya endemic to the Ecuadorian East, which is cultivated in the Palora canton, Morona Santiago provinceEl presente proyecto de investigación tiene como objetivo Caracterizar la pectina extraída de la piel de la pitahaya amarilla (Hylocereus megalanthus), procedente del cantón Palora de la finca Procel. Los análisis se realizaron en la Ciudad de Guaranda, Sector Laguacoto II, Laboratorios del Departamento de Investigación de la Universidad Estatal de Bolívar, Carrera de Ingeniería Agroindustrial. Tras los análisis físicos de las pectinas obtenidas de pitahaya amarilla en diferentes estados de madurez y tipo de ácido utilizados en este estudio, los valores de pH tanto de la pectina obtenida del T1 (estado verde + ácido cítrico) y el T2 (estado verde + ácido clorhídrico) fueron bastante similares de 3,3 a 3,4 respectivamente, de la misma manera en cuanto al T3 (estado verde + ácido cítrico) y el T4 (estado madura + ácido clorhídrico) fueron similares de 4,6 a 4,9 respectivamente. En el contenido de humedad y ceniza se obtuvo un porcentaje de 9,6 y 5,4 en cuanto al T1; de 8,4 y 5,0 para el T2; 9,2 y 5,4 para el T3: 9,7 y 5,7 en cuanto al T4. En los análisis químicos, el porcentaje del grado de esterificación de la pectina obtenida del (T1) fue de 44,4% lo cual indica que es una pectina de bajo metoxilo, mientras q las pectinas obtenidas de los tratamientos (T2, T3, y T4) el porcentaje de esterificación fue de más del 50,7% lo cual indica que estas pectinas son de alto metoxilo. El contenido de proteína de cada uno de los tratamientos fue; T1 (4,7%), T2 (7,1%), T3 (5,8%), T4 (5,2%) respectivamente, al determinar el porcentaje de ácido anhídrido Galacturonico (% AAG) el cual permite conocer el grado de pureza de la sustancia péctica; los resultados fueron similares de cada uno de los tratamientos de un 35 % de pureza de la sustancia péctica. Los análisis de Transmitancia en la pectina de piel de pitahaya amarilla; estado verde y maduro se evidencia claramente la presencia de los grupos funcionales característicos de un pectina; en la longitud de onda (λ) de 3279 se encuentra la banda correspondiente al grupo funcional –OH, para la pectina de piel de pitahaya amarilla estado verde mientras que el mismo grupo se evidencia en la pectina en estado maduro en una λ de 3336; para la λ 2918 y 2920 se encuentra la banda correspondiente al enlace C-H alifático en ambas pectinas; en la λ de 1734 se encuentra la banda correspondiente al grupo carbonilo C=O esterificados correspondiente a la pectina de piel de pitahaya amarilla estado verde, mientras que el mismo grupo carbonilo para la pectina en estado maduro se encuentra en la λ de 1731; en la λ de 1615 y 1629 se encuentra la banda correspondiente al grupo de carbonilos totales en cada una de las pectinas respectivamente y en una λ de 1014 y 1015 se encuentra la banda correspondiente a la vibración del enlace –C-O de cada una de las pectinas. Con todos estos resultados, se concluye que la pectina de piel de pitahaya en estado verde más la utilización del ácido clorhídrico, es la mejor de acuerdo a los análisis realizados, ajustándose a todos los parámetros establecidos, además es la primera vez que se extrae pectina a partir de la pitahaya amarilla eco-tipo llamado “Palora” la cual es una pitahaya endémica del Oriente ecuatoriano, que se cultiva en el cantón Palora, provincia Morona Santiago.Universidad Estatal de Bolívar . Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente . Escuela Ingeniería AgroindustrialAltuna, josé Luis2018-11-06T17:14:35Z2018-11-06T17:14:35Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://dspace.ueb.edu.ec/handle/123456789/2782spaFCCAG.IAI;111info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Estatal de Bolivarinstname:Universidad Estatal de Bolivarinstacron:UEB2024-07-27T03:02:52Zoai:dspace.ueb.edu.ec:123456789/2782Institucionalhttps://dspace.ueb.edu.ec/Universidad públicahttps://www.ueb.edu.ec/https://dspace.ueb.edu.ec/oai.Ecuador...opendoar:17352026-03-07T12:52:59.523199Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivartrue |
| spellingShingle | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) Águila Vera, Paúl Clemente PECTINA HIDROFÍLICOS ESTERIFICACIÓN NEUTRALIZACIÓN |
| status_str | publishedVersion |
| title | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| title_full | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| title_fullStr | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| title_full_unstemmed | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| title_short | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| title_sort | Caracterización de la pectina obtenida de la piel de la pitahaya amarilla (Hylocereus megalanthus) |
| topic | PECTINA HIDROFÍLICOS ESTERIFICACIÓN NEUTRALIZACIÓN |
| url | http://dspace.ueb.edu.ec/handle/123456789/2782 |