Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda.
Raw milk is a food with high nutritional value; however, its consumption without adequate thermal treatment poses a public health risk, especially in contexts with limited hygiene practices. Listeria spp. is an emerging pathogen associated with foodborne diseases (FBDs), characterized by a high mort...
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| Format: | bachelorThesis |
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2025
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| Online pristup: | https://dspace.ueb.edu.ec/handle/123456789/8747 |
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Dodaj oznaku | _version_ | 1862813017632145408 |
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| author | Pinto Cayambe, Alex Patricio |
| author2 | Mejía Fuertes, Robinson Gerardo |
| author2_role | author |
| author_facet | Pinto Cayambe, Alex Patricio Mejía Fuertes, Robinson Gerardo |
| author_role | author |
| collection | Repositorio Universidad Estatal de Bolivar |
| dc.contributor.none.fl_str_mv | Ramón Curay, Edison Riveliño |
| dc.creator.none.fl_str_mv | Pinto Cayambe, Alex Patricio Mejía Fuertes, Robinson Gerardo |
| dc.date.none.fl_str_mv | 2025-09-30T21:27:27Z 2025-09-30 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://dspace.ueb.edu.ec/handle/123456789/8747 |
| dc.language.none.fl_str_mv | es |
| dc.publisher.none.fl_str_mv | Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias. Carrera de Medicina Veterinaria. |
| dc.relation.none.fl_str_mv | FCCAG. MV; 134 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Estatal de Bolivar instname:Universidad Estatal de Bolivar instacron:UEB |
| dc.subject.none.fl_str_mv | RESISTENCIA ANTIMICROBIANA LECHE CRUDA ANTIBIÓTICOS SALUD PÚBLICA |
| dc.title.none.fl_str_mv | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Raw milk is a food with high nutritional value; however, its consumption without adequate thermal treatment poses a public health risk, especially in contexts with limited hygiene practices. Listeria spp. is an emerging pathogen associated with foodborne diseases (FBDs), characterized by a high mortality rate and increasing antimicrobial resistance. This study aimed to analyze the antimicrobial resistance profile of Listeria spp. isolated from raw milk samples sold in three markets in the Guaranda canton, Bolívar province. The research was based on the microbiological identification of the bacterium and the evaluation of its susceptibility to three commonly used antibiotics: ampicillin, ciprofloxacin, and tetracycline. A completely randomized block design (CRBD) was applied, including biochemical, microscopic, and disk diffusion (Kirby-Bauer) tests. The results revealed a prevalence of 66.67% of Listeria spp. in the analyzed samples. Susceptibility testing showed total resistance to ampicillin and tetracycline, while ciprofloxacin exhibited significant inhibitory activity. The statistical analysis (ANOVA) indicated highly significant differences between treatments (p < 0.0001), leading to the rejection of the null hypothesis and confirming significant variation in resistance profiles. These findings highlight the presence of multidrug-resistant strains in dairy products lacking sanitary control, posing a latent risk to consumers. In conclusion, the study underscores the urgent need to strengthen microbiological surveillance, regulate antibiotic use in animal production, and promote the consumption of pasteurized milk. Further studies on bacterial resistance and the implementation of comprehensive sanitary measures in the rural dairy sector are recommended. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UEB_6064eba6fd125d98bbc4d0a77f346ede |
| instacron_str | UEB |
| institution | UEB |
| instname_str | Universidad Estatal de Bolivar |
| language_invalid_str_mv | es |
| network_acronym_str | UEB |
| network_name_str | Repositorio Universidad Estatal de Bolivar |
| oai_identifier_str | oai:dspace.ueb.edu.ec:123456789/8747 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias. Carrera de Medicina Veterinaria. |
| reponame_str | Repositorio Universidad Estatal de Bolivar |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivar |
| repository_id_str | 1735 |
| spelling | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda.Pinto Cayambe, Alex PatricioMejía Fuertes, Robinson GerardoRESISTENCIA ANTIMICROBIANALECHE CRUDAANTIBIÓTICOSSALUD PÚBLICARaw milk is a food with high nutritional value; however, its consumption without adequate thermal treatment poses a public health risk, especially in contexts with limited hygiene practices. Listeria spp. is an emerging pathogen associated with foodborne diseases (FBDs), characterized by a high mortality rate and increasing antimicrobial resistance. This study aimed to analyze the antimicrobial resistance profile of Listeria spp. isolated from raw milk samples sold in three markets in the Guaranda canton, Bolívar province. The research was based on the microbiological identification of the bacterium and the evaluation of its susceptibility to three commonly used antibiotics: ampicillin, ciprofloxacin, and tetracycline. A completely randomized block design (CRBD) was applied, including biochemical, microscopic, and disk diffusion (Kirby-Bauer) tests. The results revealed a prevalence of 66.67% of Listeria spp. in the analyzed samples. Susceptibility testing showed total resistance to ampicillin and tetracycline, while ciprofloxacin exhibited significant inhibitory activity. The statistical analysis (ANOVA) indicated highly significant differences between treatments (p < 0.0001), leading to the rejection of the null hypothesis and confirming significant variation in resistance profiles. These findings highlight the presence of multidrug-resistant strains in dairy products lacking sanitary control, posing a latent risk to consumers. In conclusion, the study underscores the urgent need to strengthen microbiological surveillance, regulate antibiotic use in animal production, and promote the consumption of pasteurized milk. Further studies on bacterial resistance and the implementation of comprehensive sanitary measures in the rural dairy sector are recommended.La leche cruda constituye un alimento de alto valor nutricional, pero su consumo sin tratamiento térmico adecuado representa un riesgo para la salud pública, especialmente en contextos donde las prácticas de higiene son limitadas. Listeria spp. es un patógeno emergente asociado a enfermedades transmitidas por alimentos (ETAs), con alta tasa de mortalidad y creciente resistencia antimicrobiana. Esta investigación tuvo como objetivo analizar el perfil de resistencia antimicrobiana de Listeria spp. en muestras de leche cruda comercializadas en tres mercados del cantón Guaranda, provincia de Bolívar. El estudio se fundamentó en la identificación microbiológica de la bacteria y la evaluación de su susceptibilidad frente a tres antibióticos comúnmente utilizados: ampicilina, ciprofloxacina y tetraciclina. Se empleó un diseño experimental de bloques completamente al azar (DBCA), aplicando pruebas bioquímicas, microscópicas y de difusión en disco (Kirby-Bauer). Los resultados indicaron una prevalencia del 66,67 % de Listeria spp. en las muestras analizadas. Las pruebas de susceptibilidad revelaron resistencia total a ampicilina y tetraciclina, mientras que la ciprofloxacina mostró efecto inhibitorio significativo. El análisis estadístico (ANOVA) evidenció diferencias altamente significativas entre los tratamientos (p < 0,0001), lo que permitió rechazar la hipótesis nula y confirmar una diferencia significativa en el perfil de resistencia. Estos hallazgos alertan sobre la presencia de cepas multirresistentes en productos lácteos sin control sanitario, lo cual representa un riesgo latente para los consumidores. En conclusión, se destaca la necesidad urgente de fortalecer la vigilancia microbiológica, regular el uso de antibióticos en la producción animal y fomentar el consumo de leche pasteurizada. Asimismo, se recomienda continuar con estudios enfocados en la resistencia bacteriana y la implementación de medidas sanitarias integrales en el sector lechero rural.Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias. Carrera de Medicina Veterinaria.Ramón Curay, Edison Riveliño2025-09-30T21:27:27Z2025-09-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://dspace.ueb.edu.ec/handle/123456789/8747esFCCAG. MV; 134info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Estatal de Bolivarinstname:Universidad Estatal de Bolivarinstacron:UEB2025-11-30T18:16:34Zoai:dspace.ueb.edu.ec:123456789/8747Institucionalhttps://dspace.ueb.edu.ec/Universidad públicahttps://www.ueb.edu.ec/https://dspace.ueb.edu.ec/oai.Ecuador...opendoar:17352026-04-18T11:59:05.880957Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivartrue |
| spellingShingle | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. Pinto Cayambe, Alex Patricio RESISTENCIA ANTIMICROBIANA LECHE CRUDA ANTIBIÓTICOS SALUD PÚBLICA |
| status_str | publishedVersion |
| title | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| title_full | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| title_fullStr | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| title_full_unstemmed | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| title_short | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| title_sort | Análisis comparativo del perfil de resistencia antimicrobiana de listeria spp. en muestras de leche cruda. |
| topic | RESISTENCIA ANTIMICROBIANA LECHE CRUDA ANTIBIÓTICOS SALUD PÚBLICA |
| url | https://dspace.ueb.edu.ec/handle/123456789/8747 |