Estudio del valor proteico de la carne de cerdo mediante electroforesis
Globally, pig farming is one of the most important meat industries in agri-food production. In Ecuador, there has been sustained growth in meat consumption, with pork being the second most consumed meat after chicken. Its production is mainly distributed among three farming methods: continuous farmi...
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| Format: | bachelorThesis |
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2025
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| _version_ | 1862813010399068160 |
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| author | Arévalo Ledesma, Ambar Valeria |
| author2 | Chela Llumiguano, César Abraham |
| author2_role | author |
| author_facet | Arévalo Ledesma, Ambar Valeria Chela Llumiguano, César Abraham |
| author_role | author |
| collection | Repositorio Universidad Estatal de Bolivar |
| dc.contributor.none.fl_str_mv | Barrionuevo Mayorga, Alejandra Elizabeth |
| dc.creator.none.fl_str_mv | Arévalo Ledesma, Ambar Valeria Chela Llumiguano, César Abraham |
| dc.date.none.fl_str_mv | 2025-11-12T16:44:35Z 2025-11-12 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://dspace.ueb.edu.ec/handle/123456789/8835 |
| dc.language.none.fl_str_mv | es |
| dc.publisher.none.fl_str_mv | Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Carrera de Medicina Veterinaria |
| dc.relation.none.fl_str_mv | FCCAG-MV; 147 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Estatal de Bolivar instname:Universidad Estatal de Bolivar instacron:UEB |
| dc.subject.none.fl_str_mv | PORCICULTURA ELECTROFORESIS SDS PAGE PROTEÍNAS DUMAS POLIACRILAMIDA PM PROCEDENCIA. |
| dc.title.none.fl_str_mv | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Globally, pig farming is one of the most important meat industries in agri-food production. In Ecuador, there has been sustained growth in meat consumption, with pork being the second most consumed meat after chicken. Its production is mainly distributed among three farming methods: continuous farming, traditional farming, and free-range farming. However, there are no studies that show variations in the nutritional content, protein content, or molecular profile of the meat according to its origin. The main objective of this research was to determine the protein value of pork using vertical electrophoresis, SDS PAGE method, to obtain a detailed profile of the protein fractions according to their molecular weight (MW). Twelve types of proteins were identified, including heavy myosin, alpha actin, enolase, troponins, and light myosins. This characterization is essential both for scientific research and for industrial applications, as it enables optimization of technological processes, improvement of protein isolate functionality, and assurance of nutritional value. The results were statistically analyzed, revealing significant variations in the percentage of protein present depending on the origin. Meat from traditional farming was found to have the highest protein content, at 23.84%. In contrast, the lowest protein content was recorded in meat from continuous farming operations, with a percentage of 22.89%, suggesting a possible influence of factors such as feed management, rearing conditions, and pre- and post-slaughter stress on protein composition. These results highlight the importance of evaluating not only the type of farming, but also integrated management practices, in order to optimize the nutritional quality of pork. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UEB_797f2ffa024f3f7fe6c32413e79ab860 |
| instacron_str | UEB |
| institution | UEB |
| instname_str | Universidad Estatal de Bolivar |
| language_invalid_str_mv | es |
| network_acronym_str | UEB |
| network_name_str | Repositorio Universidad Estatal de Bolivar |
| oai_identifier_str | oai:dspace.ueb.edu.ec:123456789/8835 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Carrera de Medicina Veterinaria |
| reponame_str | Repositorio Universidad Estatal de Bolivar |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivar |
| repository_id_str | 1735 |
| spelling | Estudio del valor proteico de la carne de cerdo mediante electroforesisArévalo Ledesma, Ambar ValeriaChela Llumiguano, César AbrahamPORCICULTURAELECTROFORESISSDS PAGEPROTEÍNASDUMASPOLIACRILAMIDAPMPROCEDENCIA.Globally, pig farming is one of the most important meat industries in agri-food production. In Ecuador, there has been sustained growth in meat consumption, with pork being the second most consumed meat after chicken. Its production is mainly distributed among three farming methods: continuous farming, traditional farming, and free-range farming. However, there are no studies that show variations in the nutritional content, protein content, or molecular profile of the meat according to its origin. The main objective of this research was to determine the protein value of pork using vertical electrophoresis, SDS PAGE method, to obtain a detailed profile of the protein fractions according to their molecular weight (MW). Twelve types of proteins were identified, including heavy myosin, alpha actin, enolase, troponins, and light myosins. This characterization is essential both for scientific research and for industrial applications, as it enables optimization of technological processes, improvement of protein isolate functionality, and assurance of nutritional value. The results were statistically analyzed, revealing significant variations in the percentage of protein present depending on the origin. Meat from traditional farming was found to have the highest protein content, at 23.84%. In contrast, the lowest protein content was recorded in meat from continuous farming operations, with a percentage of 22.89%, suggesting a possible influence of factors such as feed management, rearing conditions, and pre- and post-slaughter stress on protein composition. These results highlight the importance of evaluating not only the type of farming, but also integrated management practices, in order to optimize the nutritional quality of pork.A nivel global, la porcicultura constituye una de las industrias cárnicas más relevantes dentro de la producción agroalimentaria. En Ecuador, se ha evidenciado un crecimiento sostenido en el consumo de carne, siendo la de cerdo la segunda más consumida después del pollo. Su producción se distribuye principalmente entre tres métodos de crianza: explotación continua crianza tradicional y zonas de expendio libre. Sin embargo, no existen estudios que evidencien variaciones en el contenido nutricional, proteico ni en el perfil molecular de la carne según su procedencia. El objetivo principal de la presente investigación fue determinar el valor proteico de la carne de cerdo mediante electroforesis vertical, método SDS PAGE, para obtener un perfil detallado de las fracciones proteicas según su peso molecular (PM). Se identificaron doce tipos de proteínas, entre ellas miosina pesada, alfa actina, enolasa, troponinas y miosinas ligeras. Esta caracterización resulta esencial tanto para la investigación científica como para aplicaciones industriales, ya que permite optimizar procesos tecnológicos, mejorar la funcionalidad de los aislados proteicos y garantizar su valor nutricional. Los resultados fueron analizados estadísticamente, evidenciando variaciones significativas en el porcentaje de proteínas presente según la procedencia de la carne. Se encontró que la carne proveniente de la crianza tradicional presentó el valor más alto de proteína, con un 23, 845%. En contraste, el menor contenido proteico se registró en la carne de procedencia de granjas de explotación continuas, con un porcentaje de 22,89% lo que sugiere una posible influencia de factores como el manejo alimenticio, las condiciones de crianza y el estrés pre y post sacrificio sobre la composición proteica. Estos resultados resaltan la importancia de evaluar no solo el tipo de crianza, sino también las prácticas de manejo integral, para optimizar la calidad nutricional de la carne de cerdo.Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Carrera de Medicina VeterinariaBarrionuevo Mayorga, Alejandra Elizabeth2025-11-12T16:44:35Z2025-11-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://dspace.ueb.edu.ec/handle/123456789/8835esFCCAG-MV; 147info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Estatal de Bolivarinstname:Universidad Estatal de Bolivarinstacron:UEB2025-11-30T18:11:06Zoai:dspace.ueb.edu.ec:123456789/8835Institucionalhttps://dspace.ueb.edu.ec/Universidad públicahttps://www.ueb.edu.ec/https://dspace.ueb.edu.ec/oai.Ecuador...opendoar:17352026-04-18T11:58:48.566581Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivartrue |
| spellingShingle | Estudio del valor proteico de la carne de cerdo mediante electroforesis Arévalo Ledesma, Ambar Valeria PORCICULTURA ELECTROFORESIS SDS PAGE PROTEÍNAS DUMAS POLIACRILAMIDA PM PROCEDENCIA. |
| status_str | publishedVersion |
| title | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| title_full | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| title_fullStr | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| title_full_unstemmed | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| title_short | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| title_sort | Estudio del valor proteico de la carne de cerdo mediante electroforesis |
| topic | PORCICULTURA ELECTROFORESIS SDS PAGE PROTEÍNAS DUMAS POLIACRILAMIDA PM PROCEDENCIA. |
| url | https://dspace.ueb.edu.ec/handle/123456789/8835 |