Estudio del valor proteico de la carne de cerdo mediante electroforesis

Globally, pig farming is one of the most important meat industries in agri-food production. In Ecuador, there has been sustained growth in meat consumption, with pork being the second most consumed meat after chicken. Its production is mainly distributed among three farming methods: continuous farmi...

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Auteur principal: Arévalo Ledesma, Ambar Valeria (author)
Autres auteurs: Chela Llumiguano, César Abraham (author)
Format: bachelorThesis
Publié: 2025
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Accès en ligne:https://dspace.ueb.edu.ec/handle/123456789/8835
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author Arévalo Ledesma, Ambar Valeria
author2 Chela Llumiguano, César Abraham
author2_role author
author_facet Arévalo Ledesma, Ambar Valeria
Chela Llumiguano, César Abraham
author_role author
collection Repositorio Universidad Estatal de Bolivar
dc.contributor.none.fl_str_mv Barrionuevo Mayorga, Alejandra Elizabeth
dc.creator.none.fl_str_mv Arévalo Ledesma, Ambar Valeria
Chela Llumiguano, César Abraham
dc.date.none.fl_str_mv 2025-11-12T16:44:35Z
2025-11-12
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://dspace.ueb.edu.ec/handle/123456789/8835
dc.language.none.fl_str_mv es
dc.publisher.none.fl_str_mv Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Carrera de Medicina Veterinaria
dc.relation.none.fl_str_mv FCCAG-MV; 147
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Estatal de Bolivar
instname:Universidad Estatal de Bolivar
instacron:UEB
dc.subject.none.fl_str_mv PORCICULTURA
ELECTROFORESIS
SDS PAGE
PROTEÍNAS
DUMAS
POLIACRILAMIDA
PM
PROCEDENCIA.
dc.title.none.fl_str_mv Estudio del valor proteico de la carne de cerdo mediante electroforesis
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
description Globally, pig farming is one of the most important meat industries in agri-food production. In Ecuador, there has been sustained growth in meat consumption, with pork being the second most consumed meat after chicken. Its production is mainly distributed among three farming methods: continuous farming, traditional farming, and free-range farming. However, there are no studies that show variations in the nutritional content, protein content, or molecular profile of the meat according to its origin. The main objective of this research was to determine the protein value of pork using vertical electrophoresis, SDS PAGE method, to obtain a detailed profile of the protein fractions according to their molecular weight (MW). Twelve types of proteins were identified, including heavy myosin, alpha actin, enolase, troponins, and light myosins. This characterization is essential both for scientific research and for industrial applications, as it enables optimization of technological processes, improvement of protein isolate functionality, and assurance of nutritional value. The results were statistically analyzed, revealing significant variations in the percentage of protein present depending on the origin. Meat from traditional farming was found to have the highest protein content, at 23.84%. In contrast, the lowest protein content was recorded in meat from continuous farming operations, with a percentage of 22.89%, suggesting a possible influence of factors such as feed management, rearing conditions, and pre- and post-slaughter stress on protein composition. These results highlight the importance of evaluating not only the type of farming, but also integrated management practices, in order to optimize the nutritional quality of pork.
eu_rights_str_mv openAccess
format bachelorThesis
id UEB_797f2ffa024f3f7fe6c32413e79ab860
instacron_str UEB
institution UEB
instname_str Universidad Estatal de Bolivar
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network_acronym_str UEB
network_name_str Repositorio Universidad Estatal de Bolivar
oai_identifier_str oai:dspace.ueb.edu.ec:123456789/8835
publishDate 2025
publisher.none.fl_str_mv Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Carrera de Medicina Veterinaria
reponame_str Repositorio Universidad Estatal de Bolivar
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivar
repository_id_str 1735
spelling Estudio del valor proteico de la carne de cerdo mediante electroforesisArévalo Ledesma, Ambar ValeriaChela Llumiguano, César AbrahamPORCICULTURAELECTROFORESISSDS PAGEPROTEÍNASDUMASPOLIACRILAMIDAPMPROCEDENCIA.Globally, pig farming is one of the most important meat industries in agri-food production. In Ecuador, there has been sustained growth in meat consumption, with pork being the second most consumed meat after chicken. Its production is mainly distributed among three farming methods: continuous farming, traditional farming, and free-range farming. However, there are no studies that show variations in the nutritional content, protein content, or molecular profile of the meat according to its origin. The main objective of this research was to determine the protein value of pork using vertical electrophoresis, SDS PAGE method, to obtain a detailed profile of the protein fractions according to their molecular weight (MW). Twelve types of proteins were identified, including heavy myosin, alpha actin, enolase, troponins, and light myosins. This characterization is essential both for scientific research and for industrial applications, as it enables optimization of technological processes, improvement of protein isolate functionality, and assurance of nutritional value. The results were statistically analyzed, revealing significant variations in the percentage of protein present depending on the origin. Meat from traditional farming was found to have the highest protein content, at 23.84%. In contrast, the lowest protein content was recorded in meat from continuous farming operations, with a percentage of 22.89%, suggesting a possible influence of factors such as feed management, rearing conditions, and pre- and post-slaughter stress on protein composition. These results highlight the importance of evaluating not only the type of farming, but also integrated management practices, in order to optimize the nutritional quality of pork.A nivel global, la porcicultura constituye una de las industrias cárnicas más relevantes dentro de la producción agroalimentaria. En Ecuador, se ha evidenciado un crecimiento sostenido en el consumo de carne, siendo la de cerdo la segunda más consumida después del pollo. Su producción se distribuye principalmente entre tres métodos de crianza: explotación continua crianza tradicional y zonas de expendio libre. Sin embargo, no existen estudios que evidencien variaciones en el contenido nutricional, proteico ni en el perfil molecular de la carne según su procedencia. El objetivo principal de la presente investigación fue determinar el valor proteico de la carne de cerdo mediante electroforesis vertical, método SDS PAGE, para obtener un perfil detallado de las fracciones proteicas según su peso molecular (PM). Se identificaron doce tipos de proteínas, entre ellas miosina pesada, alfa actina, enolasa, troponinas y miosinas ligeras. Esta caracterización resulta esencial tanto para la investigación científica como para aplicaciones industriales, ya que permite optimizar procesos tecnológicos, mejorar la funcionalidad de los aislados proteicos y garantizar su valor nutricional. Los resultados fueron analizados estadísticamente, evidenciando variaciones significativas en el porcentaje de proteínas presente según la procedencia de la carne. Se encontró que la carne proveniente de la crianza tradicional presentó el valor más alto de proteína, con un 23, 845%. En contraste, el menor contenido proteico se registró en la carne de procedencia de granjas de explotación continuas, con un porcentaje de 22,89% lo que sugiere una posible influencia de factores como el manejo alimenticio, las condiciones de crianza y el estrés pre y post sacrificio sobre la composición proteica. Estos resultados resaltan la importancia de evaluar no solo el tipo de crianza, sino también las prácticas de manejo integral, para optimizar la calidad nutricional de la carne de cerdo.Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Carrera de Medicina VeterinariaBarrionuevo Mayorga, Alejandra Elizabeth2025-11-12T16:44:35Z2025-11-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://dspace.ueb.edu.ec/handle/123456789/8835esFCCAG-MV; 147info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Estatal de Bolivarinstname:Universidad Estatal de Bolivarinstacron:UEB2025-11-30T18:11:06Zoai:dspace.ueb.edu.ec:123456789/8835Institucionalhttps://dspace.ueb.edu.ec/Universidad públicahttps://www.ueb.edu.ec/https://dspace.ueb.edu.ec/oai.Ecuador...opendoar:17352026-04-18T11:58:48.566581Repositorio Universidad Estatal de Bolivar - Universidad Estatal de Bolivartrue
spellingShingle Estudio del valor proteico de la carne de cerdo mediante electroforesis
Arévalo Ledesma, Ambar Valeria
PORCICULTURA
ELECTROFORESIS
SDS PAGE
PROTEÍNAS
DUMAS
POLIACRILAMIDA
PM
PROCEDENCIA.
status_str publishedVersion
title Estudio del valor proteico de la carne de cerdo mediante electroforesis
title_full Estudio del valor proteico de la carne de cerdo mediante electroforesis
title_fullStr Estudio del valor proteico de la carne de cerdo mediante electroforesis
title_full_unstemmed Estudio del valor proteico de la carne de cerdo mediante electroforesis
title_short Estudio del valor proteico de la carne de cerdo mediante electroforesis
title_sort Estudio del valor proteico de la carne de cerdo mediante electroforesis
topic PORCICULTURA
ELECTROFORESIS
SDS PAGE
PROTEÍNAS
DUMAS
POLIACRILAMIDA
PM
PROCEDENCIA.
url https://dspace.ueb.edu.ec/handle/123456789/8835