Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos
ABSTRAC Andean grains are among the foods that have been studied as a source of secondary metabolites with biological activity, which are also part of many family consumption and production systems in the Andean highlands. Through the present study it was possible to determine the presence of phytoc...
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| Fformat: | bachelorThesis |
| Iaith: | spa |
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2023
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| Mynediad Ar-lein: | http://dspace.unach.edu.ec/handle/51000/11222 |
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Ychwanegu Tag
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Ychwanegu Tag | _version_ | 1862821855266603008 |
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| author | Larrea Martínez, Andrea Mishell |
| author2 | Sánchez Tixe, Nelly Pricila |
| author2_role | author |
| author_facet | Larrea Martínez, Andrea Mishell Sánchez Tixe, Nelly Pricila |
| author_role | author |
| collection | Repositorio Universidad Nacional de Chimborazo |
| dc.contributor.none.fl_str_mv | Djabayan, Djibeyan Pablo |
| dc.creator.none.fl_str_mv | Larrea Martínez, Andrea Mishell Sánchez Tixe, Nelly Pricila |
| dc.date.none.fl_str_mv | 2023-06-30T19:49:55Z 2023-06-30T19:49:55Z 2023-06-30 |
| dc.format.none.fl_str_mv | 56p. |
| dc.identifier.none.fl_str_mv | Larrea Martínez, A y Sánchez Tixe, N(2023) Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andino. (Tesis de Pregrado) Universidad Nacional de Chimborazo, Riobamba, Ecuador. UNACH-FCS-MED http://dspace.unach.edu.ec/handle/51000/11222 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Universidad Nocional de Chimborazo |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Nacional de Chimborazo instname:Universidad Nacional de Chimborazo instacron:UNACH |
| dc.subject.none.fl_str_mv | actividad biológica hemoaglutinina anticoagulante grano andino |
| dc.title.none.fl_str_mv | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | ABSTRAC Andean grains are among the foods that have been studied as a source of secondary metabolites with biological activity, which are also part of many family consumption and production systems in the Andean highlands. Through the present study it was possible to determine the presence of phytochemicals and their biological activity in the grains of sangorache (Amaranthus hybridus L.) and chocho (Lupinus mutabilis Sweet), the results were contrasted with the information published in the specialized scientific bibliography that gives account of the finding of phytochemicals with hemagglutinating, anticoagulant, antimicrobial and cytotoxic biological activity in grains in general and particularly in Andean grains. Among the results obtained, the presence of phytoagglutinins (lectins) was demonstrated in the two Andean grains studied, through the ability of their aqueous extracts to agglutinate human erythrocytes in a non-selective manner. In addition, they have significant anticoagulant activity by acting on plasmatic coagulation proteins of both the extrinsic and intrinsic pathways. Its antimicrobial activity revealed that both aqueous extracts obtained from lupine and sangorache inhibited the bacterial growth of the ATCC gram (+) strain of Staphylococcus aureus and the gram (-) strain of Proteus mirabilis. However, the extracts did not have any antibacterial effect against E. fecalis, E. coli and K. pneumoniae. Additionally, it was verified that only the aqueous extract of the chocho, presented a percentage of toxicity of 50% on the nauplii of Artemia salina in a concentration of 1000ug/ml in 24h. Keywords: biological activity, hemagglutinin, anticoagulant, antimicrobial, cytotoxic, phytochemicals, Andean grain |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UNACH_02355a044bd64541fc8c3760d1dfd98b |
| identifier_str_mv | Larrea Martínez, A y Sánchez Tixe, N(2023) Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andino. (Tesis de Pregrado) Universidad Nacional de Chimborazo, Riobamba, Ecuador. UNACH-FCS-MED |
| instacron_str | UNACH |
| institution | UNACH |
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| publishDate | 2023 |
| publisher.none.fl_str_mv | Universidad Nocional de Chimborazo |
| reponame_str | Repositorio Universidad Nacional de Chimborazo |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazo |
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| spelling | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinosLarrea Martínez, Andrea MishellSánchez Tixe, Nelly Pricilaactividad biológicahemoaglutininaanticoagulantegrano andinoABSTRAC Andean grains are among the foods that have been studied as a source of secondary metabolites with biological activity, which are also part of many family consumption and production systems in the Andean highlands. Through the present study it was possible to determine the presence of phytochemicals and their biological activity in the grains of sangorache (Amaranthus hybridus L.) and chocho (Lupinus mutabilis Sweet), the results were contrasted with the information published in the specialized scientific bibliography that gives account of the finding of phytochemicals with hemagglutinating, anticoagulant, antimicrobial and cytotoxic biological activity in grains in general and particularly in Andean grains. Among the results obtained, the presence of phytoagglutinins (lectins) was demonstrated in the two Andean grains studied, through the ability of their aqueous extracts to agglutinate human erythrocytes in a non-selective manner. In addition, they have significant anticoagulant activity by acting on plasmatic coagulation proteins of both the extrinsic and intrinsic pathways. Its antimicrobial activity revealed that both aqueous extracts obtained from lupine and sangorache inhibited the bacterial growth of the ATCC gram (+) strain of Staphylococcus aureus and the gram (-) strain of Proteus mirabilis. However, the extracts did not have any antibacterial effect against E. fecalis, E. coli and K. pneumoniae. Additionally, it was verified that only the aqueous extract of the chocho, presented a percentage of toxicity of 50% on the nauplii of Artemia salina in a concentration of 1000ug/ml in 24h. Keywords: biological activity, hemagglutinin, anticoagulant, antimicrobial, cytotoxic, phytochemicals, Andean grainRESUMEN Los granos andinos se encuentran entre los alimentos que han sido estudiados como fuente de metabolitos secundarios con actividad biológica, que además forman parte de muchos sistemas de consumo y producción familiar en la sierra andina. Mediante el presente estudio se pudo determinar la presencia de fitoquímicos y su actividad biológica en los granos del sangorache (Amaranthus hybridus L.) y del chocho (Lupinus mutabilis Sweet), los resultados fueron contrastados con la información publicada en la bibliografía científica especializada que da cuenta del hallazgo de fitoquímicos con actividad biológica hemoaglutinante, anticoagulante, antimicrobiana y citotóxicas en los granos en general y en particular en granos andinos. Dentro de los resultados obtenidos se demostró la presencia de fitoaglutininas (lectinas) en los dos granos andinos estudiados, a través de la capacidad de sus extractos acuosos para aglutinar eritrocitos humanos de forma no selectiva. Además, presentan una importante actividad anticoagulante actuando sobre las proteínas plasmáticas de la coagulación tanto de la vía extrínseca como de la vía intrínseca. Su actividad antimicrobiana reveló que ambos extractos acuosos obtenidos del chocho y sangorache inhibieron el crecimiento bacteriano de la cepa ATCC gram (+) de Staphylococcus aureus y de la cepa gram (-) de Proteus mirabilis. Sin embargo, los extractos no tuvieron ningún efecto antibacteriano contra E. fecalis, E, coli y K. pneumoniae. Adicionalmente se constató que solo el extracto acuso del chocho, presento un porcentaje de toxicidad del 50% sobre los nauplios de Artemia salina en una concentración de 1000ug/ml en 24h.UNACH,EcuadorUniversidad Nocional de ChimborazoDjabayan, Djibeyan Pablo2023-06-30T19:49:55Z2023-06-30T19:49:55Z2023-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis56p.Larrea Martínez, A y Sánchez Tixe, N(2023) Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andino. (Tesis de Pregrado) Universidad Nacional de Chimborazo, Riobamba, Ecuador.UNACH-FCS-MEDhttp://dspace.unach.edu.ec/handle/51000/11222spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Chimborazoinstname:Universidad Nacional de Chimborazoinstacron:UNACH2023-10-03T08:15:15Zoai:localhost:51000/11222Institucionalhttp://dspace.unach.edu.ec/Universidad públicahttps://www.unach.edu.ec/http://dspace.unach.edu.ec/oai.Ecuador...opendoar:02023-10-03T08:15:15Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazofalse |
| spellingShingle | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos Larrea Martínez, Andrea Mishell actividad biológica hemoaglutinina anticoagulante grano andino |
| status_str | publishedVersion |
| title | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| title_full | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| title_fullStr | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| title_full_unstemmed | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| title_short | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| title_sort | Determinación de la actividad biológica de metabolitos fitoquímicos acuosos extraídos de granos andinos |
| topic | actividad biológica hemoaglutinina anticoagulante grano andino |
| url | http://dspace.unach.edu.ec/handle/51000/11222 |