Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería

This research was based on the use of acid cheese serum for obtaining single-cell protein through Kluyveromyces marxianus yeast. The use of whey as a by-product helps not only to reduce organic matter, but also to obtain substances with a higher added value. It is because its large amount of protein...

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Tác giả chính: Galán Robalino, Paola Andrea (author)
Định dạng: bachelorThesis
Ngôn ngữ:spa
Được phát hành: 2020
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Truy cập trực tuyến:http://dspace.unach.edu.ec/handle/51000/6996
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author Galán Robalino, Paola Andrea
author_facet Galán Robalino, Paola Andrea
author_role author
collection Repositorio Universidad Nacional de Chimborazo
dc.contributor.none.fl_str_mv Rodas Espinoza, Sonia Lourdes
dc.creator.none.fl_str_mv Galán Robalino, Paola Andrea
dc.date.none.fl_str_mv 2020-09-14T19:55:29Z
2020-09-14T19:55:29Z
2020-09-14
dc.format.none.fl_str_mv 68 p
dc.identifier.none.fl_str_mv 0263-2020 UNACH.FI.IAGRI
http://dspace.unach.edu.ec/handle/51000/6996
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Riobamba: Universidad Nacional de Chimborazo
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/ec/
info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Nacional de Chimborazo
instname:Universidad Nacional de Chimborazo
instacron:UNACH
dc.subject.none.fl_str_mv SUERO DE LECHE
PROTEÍNA UNICELULAR
LEVADURA
MINERALES
LACTEOS
BIOTECNOLOGÍA
dc.title.none.fl_str_mv Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
description This research was based on the use of acid cheese serum for obtaining single-cell protein through Kluyveromyces marxianus yeast. The use of whey as a by-product helps not only to reduce organic matter, but also to obtain substances with a higher added value. It is because its large amount of protein nutrients. There were three treatments as part of the methodology which included different types of whey such as: mozzarella cheese, fresh cheese and cottage cheese and two repetitions. The different sera used as a substrate for fermentation underwent physicochemical analyzes before and after the addition of minerals. For the protein analysis, the kjeldahl method is used. It allowed obtaining the following results: 6.95% for mozzarella cheese whey, 6.41% for cottage cheese whey, and 10.4% for fresh cheese whey (N * 6.38). The fermentation process to obtain biomass of Kluyveromyces marxianus took place at a temperature of 39 ° C. In the ash content, no differences were found, since the same amount of minerals will be placed in the three types of treatments. The acidic pH ranges from 4 to 5. It is essential for obtaining proteins, the acidity, the temperature, a correct agitation of the serum, and the use of yeasts in order to obtain a single-cell protein.
eu_rights_str_mv openAccess
format bachelorThesis
id UNACH_d12e9be36a1b904c72e7b8e33eb3919e
identifier_str_mv 0263-2020 UNACH.FI.IAGRI
instacron_str UNACH
institution UNACH
instname_str Universidad Nacional de Chimborazo
language spa
network_acronym_str UNACH
network_name_str Repositorio Universidad Nacional de Chimborazo
oai_identifier_str oai:localhost:51000/6996
publishDate 2020
publisher.none.fl_str_mv Riobamba: Universidad Nacional de Chimborazo
reponame_str Repositorio Universidad Nacional de Chimborazo
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazo
repository_id_str 0
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/ec/
spelling Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de QueseríaGalán Robalino, Paola AndreaSUERO DE LECHEPROTEÍNA UNICELULARLEVADURAMINERALESLACTEOSBIOTECNOLOGÍAThis research was based on the use of acid cheese serum for obtaining single-cell protein through Kluyveromyces marxianus yeast. The use of whey as a by-product helps not only to reduce organic matter, but also to obtain substances with a higher added value. It is because its large amount of protein nutrients. There were three treatments as part of the methodology which included different types of whey such as: mozzarella cheese, fresh cheese and cottage cheese and two repetitions. The different sera used as a substrate for fermentation underwent physicochemical analyzes before and after the addition of minerals. For the protein analysis, the kjeldahl method is used. It allowed obtaining the following results: 6.95% for mozzarella cheese whey, 6.41% for cottage cheese whey, and 10.4% for fresh cheese whey (N * 6.38). The fermentation process to obtain biomass of Kluyveromyces marxianus took place at a temperature of 39 ° C. In the ash content, no differences were found, since the same amount of minerals will be placed in the three types of treatments. The acidic pH ranges from 4 to 5. It is essential for obtaining proteins, the acidity, the temperature, a correct agitation of the serum, and the use of yeasts in order to obtain a single-cell protein.Este proyecto de investigación se fundamentó en el aprovechamiento del suero ácido de quesería para la obtención de proteína unicelular mediante el uso de la levadura Kluyveromyces marxianus. La utilización del suero como un subproducto ayuda no solamente a la disminución de materia orgánica, sino que también permite la obtención de sustancias de mayor valor agregado porque posee una gran cantidad de nutrientes proteicos. La metodología escogida fue de tres tratamientos con distintos tipos de suero: queso mozzarella, queso fresco y requesón y dos repeticiones. A los distintos sueros empleados como sustrato para la fermentación se les realizo análisis fisicoquímicos antes y después de la adición de minerales. Para el análisis de proteína se utilizó el método de kjeldahl obteniéndose los siguientes resultados: 6.95% para suero de queso mozzarella, 6.41% para suero de requesón, y 10.4% para suero de queso fresco(N*6.38). El proceso de fermentación para la obtención de biomasa de Kluyveromyces marxianus se mantuvo a una temperatura de 39°C.En el contenido de cenizas no se encontró diferencias significativas, ya que en los tres tipos de tratamientos se colocaron la misma cantidad de minerales. El pH ácido que oscila desde 4 hasta 5 ese esencial para la obtención de proteínas, la acidez la temperatura una correcta agitación del suero y uso de las levaduras lograron que se obtenga proteína unicelular.UNACH, Sede EcuadorRiobamba: Universidad Nacional de ChimborazoRodas Espinoza, Sonia Lourdes2020-09-14T19:55:29Z2020-09-14T19:55:29Z2020-09-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis68 p0263-2020 UNACH.FI.IAGRIhttp://dspace.unach.edu.ec/handle/51000/6996spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Chimborazoinstname:Universidad Nacional de Chimborazoinstacron:UNACH2022-07-11T15:23:37Zoai:localhost:51000/6996Institucionalhttp://dspace.unach.edu.ec/Universidad públicahttps://www.unach.edu.ec/http://dspace.unach.edu.ec/oai.Ecuador...opendoar:02022-07-11T15:23:37Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazofalse
spellingShingle Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
Galán Robalino, Paola Andrea
SUERO DE LECHE
PROTEÍNA UNICELULAR
LEVADURA
MINERALES
LACTEOS
BIOTECNOLOGÍA
status_str publishedVersion
title Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
title_full Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
title_fullStr Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
title_full_unstemmed Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
title_short Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
title_sort Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
topic SUERO DE LECHE
PROTEÍNA UNICELULAR
LEVADURA
MINERALES
LACTEOS
BIOTECNOLOGÍA
url http://dspace.unach.edu.ec/handle/51000/6996