Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería
This research was based on the use of acid cheese serum for obtaining single-cell protein through Kluyveromyces marxianus yeast. The use of whey as a by-product helps not only to reduce organic matter, but also to obtain substances with a higher added value. It is because its large amount of protein...
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| Định dạng: | bachelorThesis |
| Ngôn ngữ: | spa |
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2020
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| Những chủ đề: | |
| Truy cập trực tuyến: | http://dspace.unach.edu.ec/handle/51000/6996 |
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| _version_ | 1862821824837976064 |
|---|---|
| author | Galán Robalino, Paola Andrea |
| author_facet | Galán Robalino, Paola Andrea |
| author_role | author |
| collection | Repositorio Universidad Nacional de Chimborazo |
| dc.contributor.none.fl_str_mv | Rodas Espinoza, Sonia Lourdes |
| dc.creator.none.fl_str_mv | Galán Robalino, Paola Andrea |
| dc.date.none.fl_str_mv | 2020-09-14T19:55:29Z 2020-09-14T19:55:29Z 2020-09-14 |
| dc.format.none.fl_str_mv | 68 p |
| dc.identifier.none.fl_str_mv | 0263-2020 UNACH.FI.IAGRI http://dspace.unach.edu.ec/handle/51000/6996 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | Riobamba: Universidad Nacional de Chimborazo |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Nacional de Chimborazo instname:Universidad Nacional de Chimborazo instacron:UNACH |
| dc.subject.none.fl_str_mv | SUERO DE LECHE PROTEÍNA UNICELULAR LEVADURA MINERALES LACTEOS BIOTECNOLOGÍA |
| dc.title.none.fl_str_mv | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | This research was based on the use of acid cheese serum for obtaining single-cell protein through Kluyveromyces marxianus yeast. The use of whey as a by-product helps not only to reduce organic matter, but also to obtain substances with a higher added value. It is because its large amount of protein nutrients. There were three treatments as part of the methodology which included different types of whey such as: mozzarella cheese, fresh cheese and cottage cheese and two repetitions. The different sera used as a substrate for fermentation underwent physicochemical analyzes before and after the addition of minerals. For the protein analysis, the kjeldahl method is used. It allowed obtaining the following results: 6.95% for mozzarella cheese whey, 6.41% for cottage cheese whey, and 10.4% for fresh cheese whey (N * 6.38). The fermentation process to obtain biomass of Kluyveromyces marxianus took place at a temperature of 39 ° C. In the ash content, no differences were found, since the same amount of minerals will be placed in the three types of treatments. The acidic pH ranges from 4 to 5. It is essential for obtaining proteins, the acidity, the temperature, a correct agitation of the serum, and the use of yeasts in order to obtain a single-cell protein. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UNACH_d12e9be36a1b904c72e7b8e33eb3919e |
| identifier_str_mv | 0263-2020 UNACH.FI.IAGRI |
| instacron_str | UNACH |
| institution | UNACH |
| instname_str | Universidad Nacional de Chimborazo |
| language | spa |
| network_acronym_str | UNACH |
| network_name_str | Repositorio Universidad Nacional de Chimborazo |
| oai_identifier_str | oai:localhost:51000/6996 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Riobamba: Universidad Nacional de Chimborazo |
| reponame_str | Repositorio Universidad Nacional de Chimborazo |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazo |
| repository_id_str | 0 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ |
| spelling | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de QueseríaGalán Robalino, Paola AndreaSUERO DE LECHEPROTEÍNA UNICELULARLEVADURAMINERALESLACTEOSBIOTECNOLOGÍAThis research was based on the use of acid cheese serum for obtaining single-cell protein through Kluyveromyces marxianus yeast. The use of whey as a by-product helps not only to reduce organic matter, but also to obtain substances with a higher added value. It is because its large amount of protein nutrients. There were three treatments as part of the methodology which included different types of whey such as: mozzarella cheese, fresh cheese and cottage cheese and two repetitions. The different sera used as a substrate for fermentation underwent physicochemical analyzes before and after the addition of minerals. For the protein analysis, the kjeldahl method is used. It allowed obtaining the following results: 6.95% for mozzarella cheese whey, 6.41% for cottage cheese whey, and 10.4% for fresh cheese whey (N * 6.38). The fermentation process to obtain biomass of Kluyveromyces marxianus took place at a temperature of 39 ° C. In the ash content, no differences were found, since the same amount of minerals will be placed in the three types of treatments. The acidic pH ranges from 4 to 5. It is essential for obtaining proteins, the acidity, the temperature, a correct agitation of the serum, and the use of yeasts in order to obtain a single-cell protein.Este proyecto de investigación se fundamentó en el aprovechamiento del suero ácido de quesería para la obtención de proteína unicelular mediante el uso de la levadura Kluyveromyces marxianus. La utilización del suero como un subproducto ayuda no solamente a la disminución de materia orgánica, sino que también permite la obtención de sustancias de mayor valor agregado porque posee una gran cantidad de nutrientes proteicos. La metodología escogida fue de tres tratamientos con distintos tipos de suero: queso mozzarella, queso fresco y requesón y dos repeticiones. A los distintos sueros empleados como sustrato para la fermentación se les realizo análisis fisicoquímicos antes y después de la adición de minerales. Para el análisis de proteína se utilizó el método de kjeldahl obteniéndose los siguientes resultados: 6.95% para suero de queso mozzarella, 6.41% para suero de requesón, y 10.4% para suero de queso fresco(N*6.38). El proceso de fermentación para la obtención de biomasa de Kluyveromyces marxianus se mantuvo a una temperatura de 39°C.En el contenido de cenizas no se encontró diferencias significativas, ya que en los tres tipos de tratamientos se colocaron la misma cantidad de minerales. El pH ácido que oscila desde 4 hasta 5 ese esencial para la obtención de proteínas, la acidez la temperatura una correcta agitación del suero y uso de las levaduras lograron que se obtenga proteína unicelular.UNACH, Sede EcuadorRiobamba: Universidad Nacional de ChimborazoRodas Espinoza, Sonia Lourdes2020-09-14T19:55:29Z2020-09-14T19:55:29Z2020-09-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis68 p0263-2020 UNACH.FI.IAGRIhttp://dspace.unach.edu.ec/handle/51000/6996spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Chimborazoinstname:Universidad Nacional de Chimborazoinstacron:UNACH2022-07-11T15:23:37Zoai:localhost:51000/6996Institucionalhttp://dspace.unach.edu.ec/Universidad públicahttps://www.unach.edu.ec/http://dspace.unach.edu.ec/oai.Ecuador...opendoar:02022-07-11T15:23:37Repositorio Universidad Nacional de Chimborazo - Universidad Nacional de Chimborazofalse |
| spellingShingle | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería Galán Robalino, Paola Andrea SUERO DE LECHE PROTEÍNA UNICELULAR LEVADURA MINERALES LACTEOS BIOTECNOLOGÍA |
| status_str | publishedVersion |
| title | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| title_full | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| title_fullStr | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| title_full_unstemmed | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| title_short | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| title_sort | Obtención de Proteína Unicelular a partir de la Fermentación del Suero Ácido de Quesería |
| topic | SUERO DE LECHE PROTEÍNA UNICELULAR LEVADURA MINERALES LACTEOS BIOTECNOLOGÍA |
| url | http://dspace.unach.edu.ec/handle/51000/6996 |