Estudio del efecto del tamaño de partículas de la dieta en la integridad de la mucosa de los cuyes (Cavia porcellus)
Digestive integrity is directly related to the amount of nutrients absorbed and thus to the productive parameters. This research work aimed to measure the effect that particle size has on the integrity of the intestinal mucosa in (Cavia porcellus). For this purpose, 30 female A1 cockroaches, aged 15...
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| 格式: | bachelorThesis |
| 語言: | spa |
| 出版: |
2024
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| 主題: | |
| 在線閱讀: | https://dspace.unl.edu.ec/jspui/handle/123456789/29385 |
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添加標簽 | 總結: | Digestive integrity is directly related to the amount of nutrients absorbed and thus to the productive parameters. This research work aimed to measure the effect that particle size has on the integrity of the intestinal mucosa in (Cavia porcellus). For this purpose, 30 female A1 cockroaches, aged 15 days, were randomly placed in cages of 42 x 26 x 51 cm in length, width, and height, respectively. Two observation units per cage were. A completely randomized design was applied. Three diets with different particle sizes (fine, medium, and thick) were formulated taking into account the requirements of the NRC standards (1995). Each ad libitum diet was administered to 5 experimental units for 10 days. After this period, the animals were slaughtered by a strong blow to the head. Each shrimp was bled off, and a sample of 2 to 3 cm of yeyuno was taken and fixed in a 10% formol buffer solution for identification and shipping to the Histology Laboratory where PAS was dyed. (Periodic Acid Schiff). Each laboratory sample was analyzed through a microscope with a 4X increase, where each variable was analyzed: Bellis length, Lieberkühn crypt depth, muscle tunic width, and epithelial height. For each variable, 30 observations were made per sample. A variance analysis was performed using the MIXED by SAS procedure (OnDemand for Academics 2023) with the main variation factor the diet particle size. The results indicate that there was no significant difference in the variables analyzed for both animals that were fed with thick and thin particle size, however, there was a trend (p=0.062) where the thin-party-sized collars showed a 26.72% longer epithelial height than the thick particle-size animals. It is concluded that the particle size does not directly affect the length of the villi or the depth of the crypt of Lieberkühn, although it did influence the height of the epithelium. |
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