Extracción hidroalcohólica de los compuestos bioactivos del amaranto (Amaranthus spp.) en función del contenido de polifenoles y capacidad antioxidante.
This research aimed the hydroalcoholic extraction of the crude drug from amaranth, thus the plant was dehydrated in a forced air stove for 4 days at 40 ° C. The Design Expert 8.0.6 program determined 17 runs with different variables, such as: ethanol concentration (60%, 75%, 90%), extraction time (6...
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Format: | masterThesis |
Sprache: | spa |
Veröffentlicht: |
2021
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Online Zugang: | http://repositorio.utc.edu.ec/handle/27000/8125 |
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Zusammenfassung: | This research aimed the hydroalcoholic extraction of the crude drug from amaranth, thus the plant was dehydrated in a forced air stove for 4 days at 40 ° C. The Design Expert 8.0.6 program determined 17 runs with different variables, such as: ethanol concentration (60%, 75%, 90%), extraction time (6h, 15h, 24h) and temperature (30 ° C, 45 ° C, 60 ° C). According to the analysis of the phytochemical profile carried out, the metabolites it has in amaranth are: saponins, phenolic compounds, quinones / benzoquinones, flavonoids, bitter principles, mucilages, triterpenes / steroids, lactonic grouping, fatty compounds, alkaloids and catechins. The content of total polyphenols and the antioxidant capacity of the different experimental runs were determined, subjecting the crude drug to a hydroalcoholic solution. The numerical optimization of the hydroalcoholic extraction was corroborated by comparing the experimental values of total polyphenols (030.1209mg / g) and antioxidant activity (943.588µmolFe2 + / g), with those obtained from the optimization of total polyphenols (32,1006mg / g) and antioxidant activity (944.014 µmolFe2 + / g). The values achieved through experimentation are higher than the values of the numerical optimization. |
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