Caracterización de consorcios bacterianos en el cultivo de rosas (Rosa sp.) variedad Mondial®, a los 2900, 3000, 3100 msnm en Cotopaxi y Pichincha

Microorganisms are of vital importance for ecosystems, since the achievement of nutrient cycles and relationships of mutualism and antagonism in all productive and natural systems depends on them. The objective of this research was to characterize bacterial consortia present in Mondial® variety cut...

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Autore principale: Quinatoa Chasi, Kevin Ricardo (author)
Natura: bachelorThesis
Lingua:spa
Pubblicazione: 2024
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Accesso online:http://repositorio.utc.edu.ec/handle/27000/11659
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Riassunto:Microorganisms are of vital importance for ecosystems, since the achievement of nutrient cycles and relationships of mutualism and antagonism in all productive and natural systems depends on them. The objective of this research was to characterize bacterial consortia present in Mondial® variety cut roses, in three different altitudinal levels. To comply with this, it was carried out in two phases, the first consisted of collecting samples of soil, leaves and flowers of the aforementioned crop in the parishes of Guaytacama (Cotopaxi), Tabacundo (Pichincha) and Mulaló (Cotopaxi), the same ones that were They are located at an altitudinal level of 2900, 3000 and 3100 meters above sea level respectively, of which in the second phase they were isolated in a non-selective culture medium where bacterial colonies present in each of them were obtained, subsequently these were inoculated in two selective media. (Cetrimide and MacConkey) and in a general medium Mueller Hinton each with three repetitions. With the growth of the bacterial consortia in the media, morphological characterization was carried out, by optical perception, of three people, evaluating the following parameters: shape, elevation, margin, appearance, optical property, pigmentation and texture, Gram Staining was also performed. for your identification. With the data obtained, it was determined that the bacterial consortia for soil, leaves and flowers at 2900 meters above sea level presented an irregular shape (56%), shiny appearance (100%), opaque optical properties (89%), with pigmentation (78%) and smooth texture (56%). At 3000 meters above sea level, the characteristics of irregular shape (56%), shiny appearance (89%), opaque optical properties (100%) are maintained; but they differ due to the lack of pigmentation (56%) and rough texture (56%). And at 3100 meters above sea level, the irregular shape is maintained (67%), shiny appearance (100%), opaque optical property (89%), and it differs at 2900 meters above sea level with the presence of pigmentation (56%) and at 3000 meters above sea level with a smooth texture (67%).