Identificación de compuestos inhibidores que se forman en el hidrolizado enzimático de celulosa y hemicelulosa presentes en cáscara de arroz.
In the present investigation, a quantification of the inhibitory compounds that were formed in the enzymatic hydrolyzate of the cellulose and hemicellulose present in the rice husk was carried out, an accomplishment of the physical chemistry and the determination of inhibitory compounds The rice hus...
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| Médium: | bachelorThesis |
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2017
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| On-line přístup: | http://repositorio.utmachala.edu.ec/handle/48000/10074 |
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Přidat tag | Shrnutí: | In the present investigation, a quantification of the inhibitory compounds that were formed in the enzymatic hydrolyzate of the cellulose and hemicellulose present in the rice husk was carried out, an accomplishment of the physical chemistry and the determination of inhibitory compounds The rice husk, in order to know if this lignocellulosic biomass initially contains inhibitory compounds. The inhibitory compounds retard the rate of reaction of the hydrolytic enzymes, which is why the determination of these compounds can even be formed by the high temperatures or by the acidic conditions in which the enzymatic hydrolysis is carried out. These inhibitory compounds are often present upon receipt of the substrate, and others form in the hydrolyzed enzyme, during the bioconversion of cellulose by cellulase enzymes and hemicellulose by xylanase enzymes. The fungus strain that was used for the production of hydrolases enzymes that biodegraded the lignocellulosic material in its monomeric components was the fungus Trichoderma reesei, discussing by means of the studies carried out, this fungus has requirements such as the optimal temperature of oscillating growth Between 26 and 32 ° C and tolerate a wide pH range of 2 to 8.5. This fungus was inoculated in a solution of 4% carboxymethylcellulose (CMC), as the substrate of the cellulase enzyme, at a temperature of 32 ° C for a period of 24 hours, in order to adapt the fungus to the Process conditions. To achieve the general objective that is based on the quantification of the inhibitory compounds that are formed in the enzymatic hydrolyzate of cellulose and hemicellulose, a 15% solubilization of the shell in deionized water was carried out with magnetic stirring for 1 hour And followed by this we proceeded to the sample taking and the previous measurement in the visible UV spectrophotometer the presence of nitrates and nitrites. The method used for the determination of nitrates was the diazotization by and for the nitrites was the reduction of cadmium both by the visible UV spectrophotometry method. For the determination of these compounds in the enzymatic hydrolyzate, the culture medium, consisting of powdered and sifted rice husk and deionized water, was prepared at a substrate concentration of 15% which was inoculated with conidia of the fungus Trichoderma reesei, in Concentration of 2 g / L which were incubated for 72 hours at room temperature and pH 5.3-7, 3 samples were taken in a 24-hour interval between each, then the inhibitory compounds were identified Formed in the hydrolyzate. The determination of 5-HMF and levulinic acid was carried out by High Performance Liquid Chromatography, which is mass-coupled HPLC. The inhibitory compounds found in the rice husk were the nitrates that exceeded the minerals and nitrites in a lower concentration. Among the most influential compounds as inhibitors of the enzymatic hydrolysis of cellulose and hemicellulose of the rice husk were 5-HMF and levulinic acid. |
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