Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities

A field trial was conducted in two localities of the Ecuadorian Andes to evaluate potato (Solanum tuberosum c.v. INIAP - Fripapa) response to inoculation with four commercial products containing the arbuscular mycorrhizal fungus (AMF) strain Rhizophagus irregularis DAOM 197198. In parallel, potato r...

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Hlavní autor: Suarez Chacon, J. (author)
Médium: article
Vydáno: 2016
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On-line přístup:http://dspace.utpl.edu.ec/handle/123456789/18689
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author Suarez Chacon, J.
author_facet Suarez Chacon, J.
author_role author
collection Repositorio Universidad Técnica Particular de Loja
dc.creator.none.fl_str_mv Suarez Chacon, J.
dc.date.none.fl_str_mv 2016-12-20
2017-06-16T22:02:12Z
2017-06-16T22:02:12Z
dc.identifier.none.fl_str_mv 10.1007/s13199-016-0471-2
3345114
10.1007/s13199-016-0471-2
http://dspace.utpl.edu.ec/handle/123456789/18689
dc.publisher.none.fl_str_mv Symbiosis
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Técnica Particular de Loja
instname:Universidad Técnica Particular de Loja
instacron:UTPL
dc.subject.none.fl_str_mv Arbuscular mycorrhizal fungi
Commercial inoculum
LSU rRNA gene
Rhizophagus irregularis
Solanum tuberosum
dc.title.none.fl_str_mv Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description A field trial was conducted in two localities of the Ecuadorian Andes to evaluate potato (Solanum tuberosum c.v. INIAP - Fripapa) response to inoculation with four commercial products containing the arbuscular mycorrhizal fungus (AMF) strain Rhizophagus irregularis DAOM 197198. In parallel, potato roots were analysed using 454 GS-FLX+ sequencing of c. 800 bp of the nuclear LSU rRNA gene to assess the associated AMF communities. To evaluate inoculation success, sequence reads of R. irregularis on the root samples were compared between inoculated and not inoculated plants by analysing the frequency of occurrence (FO) and relative read abundance (RA). None of the commercial products significantly increased potato yield. Instead, the AMF communities were dominated by an unknown Acaulospora sp. (Sp14) found at high FO and RA in both localities. Rhizophagus irregularis was found in most of the roots of both inoculated and not inoculated plants at both localities. However, its abundance was unexpectedly low indicating poor inoculum establishment. Clearly, many factors have to be taken in consideration for the successful application of AMF-based inoculants. For the Ecuadorian field trials, several causes may explain the lack or poor establishment of R. irregularis such as inoculation technique, agricultural practices, biotic and abiotic conditions and competition with native AMF species. © 2016 Springer Science+Business Media Dordrecht
eu_rights_str_mv openAccess
format article
id UTPL_ac51f85143f9a2d44ddc07c7bb0ef0f7
identifier_str_mv 10.1007/s13199-016-0471-2
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instacron_str UTPL
institution UTPL
instname_str Universidad Técnica Particular de Loja
network_acronym_str UTPL
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oai_identifier_str oai:dspace.utpl.edu.ec:123456789/18689
publishDate 2016
publisher.none.fl_str_mv Symbiosis
reponame_str Repositorio Universidad Técnica Particular de Loja
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Técnica Particular de Loja - Universidad Técnica Particular de Loja
repository_id_str 1227
spelling Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communitiesSuarez Chacon, J.Arbuscular mycorrhizal fungiCommercial inoculumLSU rRNA geneRhizophagus irregularisSolanum tuberosumA field trial was conducted in two localities of the Ecuadorian Andes to evaluate potato (Solanum tuberosum c.v. INIAP - Fripapa) response to inoculation with four commercial products containing the arbuscular mycorrhizal fungus (AMF) strain Rhizophagus irregularis DAOM 197198. In parallel, potato roots were analysed using 454 GS-FLX+ sequencing of c. 800 bp of the nuclear LSU rRNA gene to assess the associated AMF communities. To evaluate inoculation success, sequence reads of R. irregularis on the root samples were compared between inoculated and not inoculated plants by analysing the frequency of occurrence (FO) and relative read abundance (RA). None of the commercial products significantly increased potato yield. Instead, the AMF communities were dominated by an unknown Acaulospora sp. (Sp14) found at high FO and RA in both localities. Rhizophagus irregularis was found in most of the roots of both inoculated and not inoculated plants at both localities. However, its abundance was unexpectedly low indicating poor inoculum establishment. Clearly, many factors have to be taken in consideration for the successful application of AMF-based inoculants. For the Ecuadorian field trials, several causes may explain the lack or poor establishment of R. irregularis such as inoculation technique, agricultural practices, biotic and abiotic conditions and competition with native AMF species. © 2016 Springer Science+Business Media DordrechtSymbiosis2017-06-16T22:02:12Z2017-06-16T22:02:12Z2016-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10.1007/s13199-016-0471-2334511410.1007/s13199-016-0471-2http://dspace.utpl.edu.ec/handle/123456789/18689info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Técnica Particular de Lojainstname:Universidad Técnica Particular de Lojainstacron:UTPL2017-06-16T22:02:12Zoai:dspace.utpl.edu.ec:123456789/18689Institucionalhttps://dspace.utpl.edu.ec/Institución privadahttps://www.utpl.edu.ec/https://dspace.utpl.edu.ec/oai.Ecuador...opendoar:12272017-06-16T22:02:12Repositorio Universidad Técnica Particular de Loja - Universidad Técnica Particular de Lojafalse
spellingShingle Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
Suarez Chacon, J.
Arbuscular mycorrhizal fungi
Commercial inoculum
LSU rRNA gene
Rhizophagus irregularis
Solanum tuberosum
status_str publishedVersion
title Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
title_full Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
title_fullStr Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
title_full_unstemmed Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
title_short Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
title_sort Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities
topic Arbuscular mycorrhizal fungi
Commercial inoculum
LSU rRNA gene
Rhizophagus irregularis
Solanum tuberosum
url http://dspace.utpl.edu.ec/handle/123456789/18689