Extraction and chemical characterization of two plants of popular medical use (Moringa oleifera and Petiveria alliacea) and their antimicrobial and antigenic evaluation
Traditional medicine is considered the transmission of knowledge from one generation to another linked to modifications of ancestral medicine. This transmission of knowledge has given way to the popular use of medicinal plants and their combinations to treat diseases. Moringa Oleifera, popularly kno...
محفوظ في:
| المؤلف الرئيسي: | |
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| التنسيق: | bachelorThesis |
| اللغة: | eng |
| منشور في: |
2024
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://repositorio.yachaytech.edu.ec/handle/123456789/708 |
| الوسوم: |
إضافة وسم
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إضافة وسم | الملخص: | Traditional medicine is considered the transmission of knowledge from one generation to another linked to modifications of ancestral medicine. This transmission of knowledge has given way to the popular use of medicinal plants and their combinations to treat diseases. Moringa Oleifera, popularly known as Moringa, is used as a food supplement and modulator of the immune system. Petiveria alliacea, commonly known as Anamu, is a plant used for its anti-inflammatory, anti-tumor activity and for strengthening the immune system. Previous research has confirmed the anti-inflammatory, antimicrobial, and anti-tumor activity, among others, of certain metabolites of each of these plants; however, scientific evidence is very limited regarding the humoral action of Moringa oleifera and Petiveria alliacea. This work seeks to evaluate the antigenicity of Moringa oleifera and Petiveria alliacea through immunological assays. The work was carried out in three phases: the first phase consists of the characterization and phytochemical study of the ethanolic extracts of the leaves and roots of the two plants; the second phase is based on obtaining hyperimmune sera from BALB/c mice immunized with 100 μl of each extract obtained and four immunizations were carried out to obtain the sera and their evaluation with indirect ELISA; and the third phase evaluates the antimicrobial effect of the extracts on the bacteria E. coli ATCC 25922 and S. aureus UITEY-Sa after having determined that the extracts were not cytotoxic for the SH-SY5Y cell lines. In summary, the investigation determined that extraction by the Soxhlet system provided great mass recovery performance. However, extraction by maceration provided a higher concentration of secondary metabolites. The profiles of the FTIR and HPLC techniques were similar between the E-ME and E-SE. Furthermore, the chemical characterization results between the foliar extracts of Moringa oleifera and Petiveria alliacea were similar. The evaluation of the humoral response by indirect ELISA using both extracts as antigens showed immunogenic recognition. Both extracts were non-cytotoxic, as determined by the MTT colorimetric assay. Finally, both extracts presented inhibitory activity only for the S. aureus UITEY-Sa strain. |
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