Effects of accelerated ageing and cryopreservation on seeds and embryonic axes of Phaseolus vulgaris L and Arachis hypogaea L. Germination and seedlings vigor

Description: The effects of artificial aging and cryopreservation methods on the germination of seeds and embryonic axes of Phaseolus vulgaris and Arachis hypogaea , were studied. Aging and cryopreservation treatments in bean seeds not affect the germination percentage, reaching values not significantly different from control. Germination percentage was higher when isolated embryonic axes were studied. Aging treatments gave rise to larger bean seedlings than the controls and cryopreservation treatments affected the size depending on the explant used. In groundnut seeds, aging treatments and cryopreservation positively affected germination and vigor of seedlings, but embryonic axes from not rehydrated aging seeds not germinate. Undried groundnut seeds not survive cryopreservation, while the dried ones showed a germination behavior superior to the control. Embryonic axes tolerate immersion in liquid nitrogen but when droplet-vitrification was used, formed callus instead of seedlings. Electrolyte leakage was always higher in embryonic axes than in seeds, both in beans and groundnut, and not to have a significant effect, under the conditions studied, on germination and vigor of seedlings. The present study evidences the possibility of cryopreserving, by simple procedures, germplasm of beans and groundnut, and to verify the aging tolerance of the seeds and embryonic axes of these important species.
https://peerj.com/preprints/3203/

Topic: EGUMINOSEAE
PHASEOLUS VULGARIS
ARACHIS HYPOGAEA
ARTIFICIAL AGING
CRYOPRESERVATION
GERMINATION
SEEDLINGS VIGOR
ELECTROLYTE LEAKAGE

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